Resistome, plasmidome and virulome of Enterobacterales isolated from the environment carrying mcr-like genes

• Grant number: 21/01655-7
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2021
• End date: April 30, 2024
• Field of knowledge: Biological Sciences - Microbiology
• Principal Investigator: Eliana Guedes Stehling
• Grantee: Eliana Guedes Stehling
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The first acquired mechanism of resistance to polymyxins, encoded by the mcr-1 gene, was recently described in China and, subsequently, several studies have reported the presence of mcr-like genes in different sources worldwide. The environment has been described as a reservoir of multiresistant bacteria (MDR), including Escherichia coli and antimicrobial resistance genes (ARGs), which are closely related to their spread to other sources, including humans and animals. The occurrence of mcr-like genes and other clinically relevant ARGs has already been described in bacterial isolates and in total DNA from environmental samples, however, few studies related to the plasmid characterization and genetic environments of mcr-like genes have been carried out with environmental isolates. Therefore, the objective of this research project is to characterize environmental isolates of Enterobacterales (from soil and water) carrying the mcr-like genes by sequencing the complete genome to determine the resistome, plasmidome and virulome. For that, 50 isolates will be sequenced. With the obtained results it will be possible to determine the main variants of the mcr gene that circulate in the environment, which are the main species of the order Enterobacterales carrying this gene and the complete profile of the isolates,thus contributing to the surveillance studies and the monitoring of MDR pathogens resistant to colistin, which is associated with the One Health concept. (AU)

Scientific publications

(13)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NARCIZO, JULIA PEREIRA; MANCILIO, LUCCA BONJY KIKUTI; PEDRINO, MATHEUS; GUAZZARONI, MARIA-EUGENIA; DE ANDRADE, ADALGISA RODRIGUES; REGINATTO, VALERIA. A New Pseudomonas aeruginosa Isolate Enhances Its Unusual 1,3-Propanediol Generation from Glycerol in Bioelectrochemical System. CATALYSTS, v. 13, n. 7, p. 13-pg., 2023-07-01. (21/07294-6, 21/01655-7, 21/09375-3, 22/04024-0, 21/01748-5)

FURLAN, JOAO PEDRO RUEDA; STEHLING, ELIANA GUEDES; SAVAZZI, EDUARDO ANGELINO; PEIXE, LUISA; NOVAIS, ANGELA. High occurrence of colistin- and multidrug-resistant strains carrying mcr-1 or an underestimated mcr-1.26 allelic variant along a large Brazilian river. JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, v. 30, p. 3-pg., 2022-07-01. (21/01655-7)

FURLAN, JOAO PEDRO RUEDA; SELLERA, FABIO P.; STEHLING, ELIANA GUEDES. Strengthening genomic surveillance of carbapenemases in soils: a call for global attention. LANCET MICROBE, v. 4, n. 6, p. 2-pg., 2023-06-01. (21/01655-7)

SANTOS, LUCAS DAVID RODRIGUES DOS; FURLAN, JOAO PEDRO RUEDA; ROSA, RAFAEL DA SILVA; RAMOS, MICAELA SANTANA; SAVAZZI, EDUARDO ANGELINO; STEHLING, ELIANA GUEDES. Linezolid-resistant Enterococcus casseliflavus strains of surface water and coexistence of cfr and optrA genes on an Inc18-type plasmid. JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, v. 33, p. 3-pg., 2023-03-17. (21/01655-7)

RUEDA FURLAN, JOAO PEDRO; ROSA, RAFAEL DA SILVA; RAMOS, MICAELA SANTANA; RODRIGUES DOS SANTOS, LUCAS DAVID; LOPES, RALF; SAVAZZI, EDUARDO ANGELINO; STEHLING, ELIANA GUEDES. Genetic plurality of bla(KPC-2)-harboring plasmids in high-risk clones of Klebsiella pneumoniae of environmental origin. Science of The Total Environment, v. 881, p. 8-pg., 2023-04-19. (21/01655-7, 18/01890-3)

LOPES, RALF; FURLAN, JOAO PEDRO RUEDA; RAMOS, MICAELA SANTANA; DOS SANTOS, LUCAS DAVID RODRIGUES; ROSA, RAFAEL DA SILVA; STEHLING, ELIANA GUEDES. Klebsiella quasipneumoniae subsp. similipneumoniae ST1859 O5:KL35 from Soil: First Report of qnrE1 in the Environment. MICROBIAL DRUG RESISTANCE, v. N/A, p. 5-pg., 2023-07-10. (21/01655-7)

FURLAN, JOAO PEDRO RUEDA; RIBEIRO, TERESA GONSALVES; NOVAIS, ANGELA; CABRAL, HAMILTON; PEIXE, LUISA; STEHLING, ELIANA GUEDES. Citrobacter meridianamericanus sp. nov., isolated from a soil sample. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, v. 73, n. 3, p. 5-pg., 2023-01-01. (21/01655-7, 18/01890-3, 20/14426-3)

ROSA, RAFAEL DA SILVA; FURLAN, JOAO PEDRO RUEDA; DOS SANTOS, LUCAS DAVID RODRIGUES; RAMOS, MICAELA SANTANA; SAVAZZI, EDUARDO ANGELINO; STEHLING, ELIANA GUEDES. Early dissemination of novel NDM-1-producing Klebsiella pneumoniae ST6326 to the environment. JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, v. 34, p. 2-pg., 2023-09-01. (21/01655-7, 18/01890-3)

FURLAN, JOAO PEDRO RUEDA; STEHLING, ELIANA GUEDES. Predicting tigecycline susceptibility in multidrug-resistant Klebsiella species and Escherichia coli strains of environmental origin. Brazilian Journal of Microbiology, v. N/A, p. 7-pg., 2023-06-16. (21/01655-7)

LOPES, RALF; FURLAN, JOAO PEDRO RUEDA; STEHLING, ELIANA GUEDES. Acquisition of bla(IMP-13) on a novel IncP-7 plasmid in XDR VIM-2-positive Pseudomonas aeruginosa belonging to the global high-risk clone ST235 in an agricultural ecosystem. JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, v. 30, p. 3-pg., 2022-08-22. (18/01890-3, 21/01655-7)

FURLAN, JOAO PEDRO RUEDA; RAMOS, MICAELA SANTANA; ROSA, RAFAEL DA SILVA; SAVAZZI, EDUARDO ANGELINO; STEHLING, ELIANA GUEDES. Occurrence and genetic characteristics of multidrug-resistant Escherichia coli isolates co-harboring antimicrobial resistance genes and metal tolerance genes in aquatic ecosystems. INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH, v. 244, p. 8-pg., 2022-06-29. (18/01890-3, 21/01655-7)

LOPES, RALF; FURLAN, JOAO PEDRO RUEDA; DOS SANTOS, LUCAS DAVID RODRIGUES; STEHLING, ELIANA GUEDES. Detection of CTX-M-27-positive endophytic Escherichia coli ST131 lineage C1/H30R subclade carrying bla(KPC-2) on an IncX3-IncU plasmid in a fresh vegetable. JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, v. 30, p. 2-pg., 2022-07-11. (18/01890-3, 21/01655-7)

NAKAZATO, GERSON; BELLO-TOLEDO, HELIA MAGALY; ZHANG, ANYUN; STEHLING, ELIANA GUEDES. Editorial: Molecular Characterization of Clinically Important Gram-Negative Bacteria Recovered From the Environment: Antimicrobial Resistance, Virulence and Epidemiology. FRONTIERS IN MICROBIOLOGY, v. 13, p. 3-pg., 2022-07-08. (21/01655-7)