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Influence of cigarrete smoke inalation in apical periodontitis development: local and sistemic inflamatory anallisys in Wistar rats

Grant number: 20/06310-5
Support Opportunities:Regular Research Grants
Duration: November 01, 2021 - October 31, 2023
Field of knowledge:Health Sciences - Dentistry - Endodontics
Principal Investigator:Eloi Dezan Junior
Grantee:Eloi Dezan Junior
Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
Associated researchers: Carlos Roberto Emerenciano Bueno ; Claudia Cristina Biguetti ; Luciano Tavares Angelo Cintra ; Marco Antonio Hungaro Duarte ; Marina Tolomei Sandoval Cury ; Mariza Akemi Matsumoto


Endodontic infection occurs mainly after contamination of the pulp tissue, leading to bacterial colonization of root canals, resulting in an inflammatory response of the periapical tissues and the formation of a periapical lesion, an Apical Periodontitis (AP). Smoking is considered a major risk factor for oral infections, studied mainly in periodontal disease, as it affects gingival tissues, periodontal ligament, vascular system, inflammatory response and bone metabolism. Observing into account these systemic changes in the technical response, the aim of this study will be to evaluate in Wistar rats the influence of smoking on the inflammatory profile of AP. Forty animals will be divided into 4 groups (n = 10): AP (rats with induced apical periodontitis); S (rats exposed to cigarette smoke); SAP (mice with induced apical periodicity and exposure to cigarette smoke); Control (rats without PA and without exposure to cigarettes). For exposure to cigarette smoke, the animals stay in a smoking chamber for 8 minutes, three times a day. The animals will be exposed to cigarette smoke 20 days before the induction of PA. For PA induction, as pulps of the upper and lower first molars, the rights will be exposed to the oral environment for 30 days, during which time the exposure of cigarette smoke will continue, totaling 50 days of exposure to smoke. Hematologic parameters will be analyzed, calcium, phosphorus and alkaline phosphatase as bone markers, nicotine and cotinine will be analyzed. After euthanasia, mandible and maxillae will be removed and mandible will be used to evaluate the volume of bone destruction in apical periodontitis by micro CT. Then, samples will be embedded in paraffin to assess the inflammatory profile via hematoxylin-eosin staining for morphological and histomorphometric analysis (characterization and quantification of inflammatory cells), macrophage detection via immunohistochemistry. Interleukin IL-6, IL-1² e TNF-± as local inflammation indicators; RANKL e OPG as bone resorption indicator, and evaluation of collagen fibers of periodontal ligament by Picrosirius red birefringence. The data obtained will be tabulated and submitted to statistical analysis with a significance level of 5%. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DA SILVA, ANA CLAUDIA RODRIGUES; VASQUES, ANA MARIA VEIGA; BUENO, CARLOS ROBERTO EMERENCIANO; ERVOLINO, EDILSON; CINTRA, LUCIANO TAVARES ANGELO; BIGUETTI, CLAUDIA CRISTINA; MATSUMOTO, MARIZA AKEMI; DEZAN-JUNIOR, ELOI. Effects of cigarette smoke inhalation on the immune-inflammatory profile of experimental apical periodontitis in rats. International Endodontic Journal, v. N/A, p. 12-pg., . (20/06310-5)
VASQUES, ANA MARIA VEIGA; DA SILVA, ANA CLAUDIA RODRIGUES; BUENO, CARLOS ROBERTO EMERENCIANO; CURY, MARINA TOLOMEI SANDOVAL; ERVOLINO, EDILSON; CINTRA, LUCIANO TAVARES ANGELO; DEZAN JUNIOR, ELOI. Inflammatory profile of apical periodontitis exacerbated by cigarette smoke inhalation: Histological and immunohistochemical analysis in rats. International Endodontic Journal, v. N/A, p. 10-pg., . (20/06310-5)

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