Study of the bone dynamics of an implant surface coated with odanacatib through wettability, topographical, biomechanical, computed microtomography, histomorphometric, biocompatibility, culture of cells and immunohistochemistry

• Grant number: 21/08538-6
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2022
• End date: January 31, 2024
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Idelmo Rangel Garcia Junior
• Grantee: Idelmo Rangel Garcia Junior
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
• Associated researchers: Francisley Ávila Souza ; Reinhard Gruber ; Roberta Okamoto ; Sandra Helena Penha de Oliveira
• Associated scholarship(s): 22/06429-8 - Study of bone response of implant surface coated with odanacatib through wettability, topographic, cell culture, histomorphometry and computed microtomography analyses, BP.TT

Abstract

Currently, studies found in the literature promote the success of osseointegration related to surface treatment of titanium implants. Addressing the challenges of finding chemical components that promote changes in favor of the bone neoformation process. However, there is still a need to improve the technology of these materials for early bone regeneration. Thus, the objective of this work is to evaluate the dynamics of the bone response of a surface modified by acid etching, modified by acid plus Body Fluid Solution (SBF), and modified by the active ingredient Odanacatib, at a concentration of 0.86mg / L, by the biomimetic method in a standardized rat tibia model. For this, 144 male rats (Rattus Copernicus Albinus Wistar) and 288 commercially pure titanium implants of 1.6 in diameter and 3.0 mm in height will be available, 96 with double acid etch surface (GC), 96 with surface modified by double acid etching, and SBF (GX) 96 with modified surface based on Odanacatib (GO) by the biomimetic method of surface modification that will be installed on the tibiae of rats. The periods of euthanasia for the collection of tibias will be 15 and 40 days. For an analysis of cell culture used, 18 male Wistar rats (Rattus Copernicus Albinus Wistar) and 108 titanium disks of 0.5 cm in diameter and 0.3 cm in height will be used for 24 wells, 36 with double surface acid etching (GCC), 36 with surface modified by SBF (GXC) and 36 with surface modified based on Odanacatib (GOC) by the biomimetic method of surface modification. For a subcutaneous analysis, 48 rats (Rattus Copernicus Albinus Wistar) will be exposed and 96 titanium discs of 0.5 cm in diameter and 0.3 cm in height will be used, 32 with double acid etch surface (GCS), 32 with surface modified by SBF (GXS) and 32 with surface modified based on Odanacatib (GOS) by the biomimetic method of surface modification that will be installed 2 discs per animal in the subcutaneous tissue in the extreme regions of the animal's back, in which tissue collection subcutaneous will be 3 and 7 days. The specimens will be decomposed to the analysis of wettability, topography, cell culture, biomechanics, computerized microtomography, histology, histometry, and immunohistochemistry. Finally, the quantitative data of the studied groups will be collected through a statistical analysis, adopting the significance level p <0.05. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANCHES, NATALIA DOS SANTOS; PANAHIPOUR, LAYLA; WANG, LEI; IMANI, ATEFE; MARCHIOLLI, CAROLINE LIBERATO; CERVANTES, LARA CRISTINA CUNHA; STEIN, MARIA CRISTINA RUIZ VOMS; BERTON, SARA ALVES; SOUZA, FRANCISLEY AVILA; OKAMOTO, ROBERTA; et al. Cytokine expression of soft tissue cells cultured with titanium discs and their respective supernatants in vitro. CLINICAL ORAL INVESTIGATIONS, v. 29, n. 1, p. 18-pg., 2025-01-14. (21/08067-3, 21/08538-6)