Infertility related to endometriosis in a murine model

• Grant number: 21/13512-6
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2022
• End date: February 29, 2024
• Field of knowledge: Health Sciences - Medicine - Maternal and Child Health
• Principal Investigator: Paula Andrea de Albuquerque Salles Navarro
• Grantee: Paula Andrea de Albuquerque Salles Navarro
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated researchers: Alceu Afonso Jordão Junior ; Caroline Mantovani da Luz ; Julio Cesar Rosa e Silva ; Nathalia Franzoni Smargiassi

Abstract

Recently, a murine model of infertility related to endometriosis was developed. The infertility was evaluated for 1 to 2 months after the surgical procedure to induce experimental endometriosis. To find out if this model can be used in studies of therapeutic intervention, it is important to test for a longer period. Thus, we aim to investigate whether the transplantation of fragments of endometrial tissue in the peritoneal wall of mice promotes experimental endometriosis and reduced natural fertility for 4 and 12 weeks after the surgical procedure. For this, an experimental study will be developed in which the animals will be divided into 2 groups: control group (simulated surgery) and endometriosis group 1 (peritoneal transplantation of 2 fragments of endometrial tissue of 5 mm each), breed with fertile males after 4 and 12 weeks of the procedure. The animals that impregnate will be euthanized after the weaning and the non-pregnant ones, after 4 weeks with the males. The duration of pregnancy, the litter size, the weights of the litter, the serum levels of oxidative stress markers (8-hydroxy-2'-deoxyguanosine and total antioxidant capacity) and the histological analysis of tissue from the implant sites will be compared between animals of the two experimental groups. If is characterized a persistence in the effect of surgically induced endometriosis in the reduction of fertility, it will be possible to use this model as translational in research studies for new treatments to improve the natural fertility of patients with infertility related to endometriosis. (AU)