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In vivo study of the inflammatory reactivity of bacterial cellulose nanomembrane experimentally implanted in the peritoneum of Wistar rats

Grant number: 22/01614-1
Support Opportunities:Regular Research Grants
Start date: June 01, 2022
End date: May 31, 2024
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Pathology
Principal Investigator:Alessandre Hataka
Grantee:Alessandre Hataka
Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
Associated researchers:Angela Faustino Jozala ; Claudio Gustavo Barbeito ; Denise Grotto ; Luciana Pinato

Abstract

Traumatic injuries involving fraying and tissue loss in domestic animals are frequent, and in many situations, tissue healing, recovery, or reconstruction is not possible due to its extension, and requires the use of bioimplants. Bacterial Cellulose (BC) has been used for various applications in human medicine. However, in the veterinary field, studies investigating the morphological and immunohistochemical characteristics of the inflammatory and healing component in BC implanted in the peritoneum in vivo were not observed. Even today, there is controversy over whether or not BC is biocompatible with regard to the inflammatory and healing response. The objective of this work will be to evaluate the safety/systemic toxicity generated by the BC membrane implanted intraperitoneally in Wistar rats, through biochemical analyses, oxidative stress, histopathological and immunohistochemical analysis of biological phenomena at the BC implant site. For that, 50 male or female Wistar rats will receive surgically implanted BC in the peritoneal cavity, 25 animals will receive BC and 25 will be the sham group. After 60 postoperative days, blood, serum, plasma, liver and kidneys will be collected for biochemical and oxidative stress tests and the implant site, heart, liver and kidneys will be collected for histopathological and immunohistochemical analysis. The inflammatory and healing response around the BC will be evaluated, as well as the intensity and type of inflammatory infiltrate by histopathology. Through immunohistochemistry, the types of lymphocytes, T (CD3) or B (CD20) and macrophages ( MCA 874G) in the process and the type and amount of collagen fibers, I, III and IV, as well as the presence of myofibroblasts (alpha SMA) will be evaluated. The results obtained will be spread out and statistically analyzed to verify whether or not there are significant differences. (AU)

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VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)