Synthetic communities of the citrus rhizosphere microbiota seeking benefits for plant development and resistance to stress

• Grant number: 20/14584-8
• Support Opportunities: Research Projects - Thematic Grants
• Start date: June 01, 2022
• End date: May 31, 2027
• Field of knowledge: Agronomical Sciences - Agronomy
• Principal Investigator: Helvecio Della Coletta Filho
• Grantee: Helvecio Della Coletta Filho
• Host Institution: Instituto Agronômico (IAC). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). Campinas , SP, Brazil
• Pesquisadores principais: Marco Aurélio Takita
• Associated researchers: Alessandra Alves de Souza ; Carolina Sardinha Francisco ; Dirceu de Mattos Junior ; Jaderson Silveira Leite Armanhi ; Juliana Erika de Carvalho Teixeira Yassitepe ; Mariângela Cristofani-Yaly ; Paulo Arruda ; Rafael Soares Correa de Souza ; Raquel Luciana Boscariol Camargo ; Sérgio Alves de Carvalho
• Associated scholarship(s): 25/02825-4 - Responses to inoculations of synthetic rhizobacterial communities (Ci-SynCom) in citrus, BP.TT; 24/00651-6 - Beneficial microorganisms as strategies to maximize growth and resilience of Citrus., BP.JC; 24/00667-0 - Beneficial microorganisms as strategies to maximize growth and resilience of Citrus., BP.JC; + associated scholarships 23/13314-5 - Exploring the rhizospheric microbiota of Citrumelo Swingle rootstock in the field under water stress conditions, BP.MS; 23/01392-1 - Characterization of citrus rhizobacterial populations under organic and conventional cultivation systems, BP.MS; 23/03131-0 - Identification and selection of synthetic communities of rhizobacteria from citrus plant roots., BP.PD - associated scholarships

Abstract

The nursery phase is an important step in the citrus industry. For the citrus plant, the rootstock has a significant influence on physiological responses of the canopy such as biotic and abiotic stress, plant architecture, and fruit quality. Rootstocks that impact plant size architecture bring advantages to fruit quality, management, pest inspections, and harvest. However, this kind of rootstock is little used by the growers because of the low initial growth rate in the nursery steps and in the field. Despite the unquestionable phytosanitary quality of citrus nursery plants produced under screen-house in SP, the substrates (coconut fiber or pine bark) regularly used for plant growth are unknown regarding the presence of microorganisms beneficial for plant rhizosphere. The reconstruction of the rhizosphere microbiota, with benefits on the increase of plant growth and favorable response to different stress conditions, has been investigated in several plant species with very promising results. The main objective of this proposal is to establish a rhizobacterial community of citrus field trees, based on previous knowledge of our research group (DOI 10.1038/s41467-018-07343-2), as well as to characterize as an inductor of citrus plants growth and antagonistic effect on pathogens of interest in the citrus production system and hydric stress. The subprojects will explore the bacteria community on citrus rhizosphere, isolation and definition of synthetic communists - SynCom (Subproj. 1); advantageous effects of Citrus-SynComs on the plants (Subproj. 2); capacity of colonization and prevalence of inoculated Ci-SynComs (Subproj, 3); response of Ci-SynCom-inoculated plants to biotic and abiotic stress (Subproj, 4 and 5); roots architecture (Subproj. 6); and performance of Ci-SynCom inoculated plants in the commercial nursery (Subproj. 7). In the end, it is expected to establish a product (Ci-SynCom) to be used in the nursery production system with the objective of improving growth and responses to abiotic and biotic stresses of the plants, with benefits after planting in the field. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LIMA, HELENA SANTIAGO; MANCINE, NATHALIA; PERUCHI, GIOVANA BETIN; FRANCISCO, CAROLINA SARDINHA; WANG, NIAN; DE SOUZA, RAFAEL SOARES CORREA; ARMANHI, JADERSON SILVEIRA LEITE; COLETTA-FILHO, HELVECIO DELLA. Microbial community of cultivated and uncultivated citrus rhizosphere microbiota in Brazil. SCIENTIFIC DATA, v. 11, n. 1, p. 8-pg., 2024-11-27. (20/14584-8, 23/13314-5, 23/03131-0, 23/01392-1)