Development of new kits destined for the diagnosis of intestinal parasites in feces samples

• Grant number: 99/06228-4
• Support Opportunities: Research Grants - Innovative Research in Small Business - PIPE
• Start date: May 01, 2000
• End date: November 30, 2003
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal Investigator: Sumie Hoshino Shimizu
• Grantee: Sumie Hoshino Shimizu

Abstract

The project envisions the development of kits for the diagnosis of intestinal parasites in feces samples. The kits may be industrialized and sold by the company Immunoassay Produtos Hospitalares Ltda. Recent data released by the World Health Organization shows that close to 3.5 billion people in the world find themselves with intestinal parasites, of which 450 million are ill, the majority being children living in tropical areas, including Brazil. Market research indicates that there are few national diagnostic kits destined towards the examination of feces. This project is divided into three sub-projects. The first deals with the development of a simple, practical and lowcost kit, which will permit the collection of feces, concentrating on the parasitic elements and their morphological identification through the optical microscope, which could, at the next stage, be linked to an automatic system. The two other kits characterize themselves as being diagnostically advanced, for use with monoclonic antibodies and the technique of genomic amplification. In this manner, the second kit will be destined to the detection of antigens of Entamoeba histolytica, by way of immuno enzyme testing (Elisa). The third kit will be for the detection of Cryptosporidium parvum, Cyclospora cayetanensis and Microsporida, opportunist and emergent protozoa that are present under immune deficiency conditions, such as those observed with AIDS. This kit is based on the amplification of DNA, using a system of biotinylation primers, and the identification of the amplified products on plastic trays treated with avidin, internal marking probes with digoxygenin, and enzyme conjugates anti-digoxygenin (PCR- colorimetry). Kits for the detection of C. parvumcould also be applied for the analysis of water quality. The present project could open up paths towards the standardization, evaluation and the production of other immune reagents applied in the diagnosis of the other intestinal protozoa, among them the giardiasis extra-intestinal infections by free living amebas. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

INACIO, SANDRA VALERIA; GOMESB, JANCARLO FERREIRA; MIRANDA OLIVEIRA, BRUNO CESAR; FALCAO, ALEXANDRE XAVIER; NAGASE SUZUKI, CELSO TETSUO; DOS SANTOS, BIANCA MARTINS; COSTA DE AQUINO, MONALLY CONCEICAO; DE PAULA RIBEIRO, RAFAELA SILVA; DE ASSUNCAO, DANILLA MENDES; FREIRE CASEMIRO, PAMELLA ALMEIDA; et al. Validation of a new technique to detect Crytosporidium spp. oocysts in bovine feces. PREVENTIVE VETERINARY MEDICINE, v. 134, p. 1-5, NOV 1 2016. (99/06228-4)