Functionalized solution-processed 2D materials: development of prototyping electric sensors and biosensors

• Grant number: 21/06238-5
• Support Opportunities: Research Grants - Young Investigators Grants
• Start date: February 01, 2023
• End date: January 31, 2028
• Field of knowledge: Engineering - Materials and Metallurgical Engineering - Nonmetallic Materials
• Principal Investigator: Rafael Furlan de Oliveira
• Grantee: Rafael Furlan de Oliveira
• Host Institution: Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia e Inovação (Brasil). Campinas , SP, Brazil
• Associated researchers: Adalberto Fazzio ; Ana Carolina Migliorini Figueira ; Carlos César Bof Bufon
• Associated research grant(s): 23/09395-0 - Multiuser equipment (EMU) granted in the project 2021/06238-5: Keihtley 4200A semiconductor characterization system, AP.EMU
• Associated scholarship(s): 24/12530-9 - Electrolytic Transistors Based on 2D Materials for Sensing, Biosensing and Bioelectronics, BP.DD; 24/01961-9 - Functionalized graphene oxide (GO) and reduced graphene oxide (rGO): Effect on the AC and DC electrical transduction of bio-interactions, BP.MS; 23/03501-2 - Evaluation of the Operational Characteristics and Stability of Liquid-gated Transistors based on Reduced Graphene Oxide in Artificial Fluids for Biosensing Applications, BP.IC

Abstract

In this project, we propose the development of solid knowledge and resulting prototyping device technology based on the functionalization of solution-processed 2D materials (2DMs) and their integration "on chip" for chemical sensing and biosensing. The use of 2DMs present unique advantages for this purpose, such as i) high surface-to-volume ratio, which make them very sensitive to environmental perturbations; ii) adjustable electrical properties, which guarantees flexibility during the device operation; and iii) inherent structural defects that can be usefully functionalized via defect engineering. Solution processability allows the production of 2DM "inks" which are compatible with industrial scalable manufacturing methods, enabling device prototyping. Here, by the ad hoc functionalization of solution-processed 2DMs with chemical and biochemical recognition elements (viz., functional molecules and biomolecules), we will develophighly sensitive and selective sensors and biosensors using transducers based on electrolytegated transistors. As kick-off applications, we will develop point-of-care (PoC) sensors and biosensors beyond proof-of-concept and with applicability in healthcare. For chemical sensing, we envisage the detection of metal cations (K+, Na+, Ca2+, e Mg2+) in physiological samples, such as saliva, sweat, urine and blood, whose concentration can be directly correlated to several pathologies. For biosensing, we aim for the monitoring of antibodies related to autoimmune inflammatory pathologies, such as rheumatoid arthritis. By tackling these two initial applications, we intend to demonstrate how inherent defects of 2DMs can be functionalized to develop novelPoC (bio)sensors endowed of high sensitivity, selectivity, and with real potential for prototyping and commercialization. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OKAZAKI, ANDERSON K.; DE OLIVEIRA, RAFAEL FURLAN; FREIRE, RAFAEL LUIZ HELENO; FAZZIO, ADALBERTO; DE LIMA, FELIPE CRASTO. Uncovering the Structural Evolution of Arsenene on SiC Substrate. Journal of Physical Chemistry C, v. 127, n. 16, p. 6-pg., 2023-04-13. (17/02317-2, 21/06238-5, 19/14949-9, 19/20857-0, 20/14067-3)

SENSI, MATTEO; DE OLIVEIRA, RAFAEL FURLAN; BERTO, MARCELLO; PALMIERI, MARINA; RUINI, EMILIO; LIVIO, PIETRO ANTONIO; CONTI, ANDREA; PINTI, MARCELLO; SALVARANI, CARLO; COSSARIZZA, ANDREA; et al. Reduced Graphene Oxide Electrolyte-Gated Transistor Immunosensor with Highly Selective Multiparametric Detection of Anti-Drug Antibodies. Advanced Materials, v. 35, n. 36, p. 9-pg., 2023-07-28. (21/06238-5)

SENSI, MATTEO; DE OLIVEIRA, RAFAEL FURLAN; BERTO, MARCELLO; PARADISI, ALESSANDRO; GRECO, PIERPAOLO; BORTOLOTTI, CARLO AUGUSTO; SAMORI, PAOLO; BISCARINI, FABIO. How Biorecognition Affects the Electronic Properties of Reduced Graphene Oxide in Electrolyte-Gated Transistor Immunosensors. ADVANCED FUNCTIONAL MATERIALS, v. 34, n. 19, p. 14-pg., 2024-01-14. (21/06238-5)

SELMI, GUILHERME SEGOLIN; LOURENCO NETO, EDUARDO RODRIGUES; LELIS, GABRIELLE COELHO; OKAZAKI, ANDERSON KENJI; RIUL JR, ANTONIO; BRAUNGER, MARIA LUISA; DE OLIVEIRA, RAFAEL FURLAN. Pulse Dynamics in Reduced Graphene Oxide Electrolyte-Gated Transistors: Charge Memory Effects and Mechanisms Governing the Ion-To-Electron Transduction. ADVANCED ELECTRONIC MATERIALS, v. N/A, p. 12-pg., 2024-12-17. (21/06238-5, 23/03501-2, 19/14949-9)

DE LIMA, ALESSANDRO HENRIQUE; SCARPA, ISABELLA; AZEVEDO, NATHALIA CRISTINE LIMA; LELIS, GABRIELLE COELHO; STRAUSS, MATHIAS; MARTINEZ, DIEGO STEFANI TEODORO; DE OLIVEIRA, RAFAEL FURLAN. Oxidative debris in graphene oxide: a decade of research. JOURNAL OF MATERIALS CHEMISTRY C, v. N/A, p. 24-pg., 2023-09-05. (23/03501-2, 21/06238-5, 19/14949-9)

NOZELLA, NATAN LUIS; LIMA, JOAO VICTOR MORAIS; DE OLIVEIRA, RAFAEL FURLAN; GRAEFF, CARLOS FREDERICO DE OLIVEIRA. Melanin/PEDOT:PSS blend as organic mixed ionic electronic conductor (OMIEC) for sustainable electronics. MATERIALS ADVANCES, v. N/A, p. 12-pg., 2023-09-13. (22/00410-3, 21/03379-7, 13/07296-2, 20/12356-8, 21/06238-5)

DE LIMA, ALESSANDRO HENRIQUE; SCARPA, ISABELLA; AZEVEDO, NATHALIA CRISTINE LIMA; LELIS, GABRIELLE COELHO; STRAUSS, MATHIAS; MARTINEZ, DIEGO STEFANI TEODORO; DE OLIVEIRA, RAFAEL FURLAN. Oxidative debris in graphene oxide: a decade of research. JOURNAL OF MATERIALS CHEMISTRY C, v. 11, n. 37, p. 25-pg., 2023-09-28. (23/03501-2, 21/06238-5, 19/14949-9)

LELIS, GABRIELLE COELHO; FONSECA, WILSON TIAGO; DE LIMA, ALESSANDRO HENRIQUE; OKAZAKI, ANDERSON KENJI; FIGUEIREDO, EDUARDO COSTA; RIUL JR, ANTONIO; SCHLEDER, GABRIEL RAVANHANI; SAMORI, PAOLO; DE OLIVEIRA, RAFAEL FURLAN. Harnessing Small-Molecule Analyte Detection in Complex Media: Combining Molecularly Imprinted Polymers, Electrolytic Transistors, and Machine Learning. ACS APPLIED MATERIALS & INTERFACES, v. N/A, p. 11-pg., 2023-12-22. (23/03501-2, 21/06238-5, 19/14949-9)

NOZELLA, NATAN L.; SELMI, GUILHERME S.; GUERRA, NAYRIM B.; PITON, MARCELO R.; GRAEFF, CARLOS F. O.; DE OLIVEIRA, RAFAEL F.. Melanin/PEDOT:PSS organic synaptic transistors: a step towards sustainable neuromorphic applications. NEUROMORPHIC COMPUTING AND ENGINEERING, v. 5, n. 1, p. 8-pg., 2025-03-01. (22/00410-3, 13/07296-2, 21/06238-5, 21/09207-3)

NOZELLA, NATAN L.; PAULIN, JOAO V.; NOGUEIRA, GABRIEL L.; GUERRA, NAYRIM B.; DE OLIVEIRA, RAFAEL F.; GRAEFF, CARLOS F. O.. Probing n-Type Conduction in Eumelanin Using Organic Electrochemical Transistors. ACS APPLIED ELECTRONIC MATERIALS, v. 7, n. 8, p. 6-pg., 2025-04-03. (21/06238-5, 22/00410-3, 22/10998-8, 13/07296-2, 21/09207-3)