Diversity and identification of final larval stages of marine shrimp (Dendrobranchiata: Penaeoidea) from plankton off the coast of São Paulo through morphology and DNA barcoding

• Grant number: 23/01382-6
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2023
• End date: June 30, 2025
• Field of knowledge: Biological Sciences - Zoology - Morphology of Recent Groups
• Agreement: CNPq
• Principal Investigator: Rogério Caetano da Costa
• Grantee: Rogério Caetano da Costa
• Host Institution: Faculdade de Ciências (FC). Universidade Estadual Paulista (UNESP). Campus de Bauru. Bauru , SP, Brazil
• Associated research grant: 18/13685-5 - Integrative analysis of the Brazilian fauna of decapod crustaceans: taxonomy, phylogenetic systematics, spermiotaxonomy, morphology of post-embryonic development, ecology and conservation, AP.BTA.TEM
• Associated scholarship(s): 23/10796-9 - Diversity and identification of final larval stages of marine shrimp (Dendrobranchiata: Penaeoidea) from plankton off the coast of São Paulo through morphology and DNA barcoding, BP.JD

Abstract

Studies in the state of São Paulo has mainly focused the juvenile and adult of Dendrobranchiata marine shrimp, significantly expanding the knowledge of this group. Penaeoidea has five families with 388 worldwide recognized species, Aristeidae (26), Penaeidae (229), Sicyoniidae (52) and Solenoceridae (81), most of which are benthic and marine; many of them has ecological and commercial interest. There are three planktonic larval stages, nauplius, zoea (protozoea and mysis) and decapodite (also called postlarvae in penaeids); depending on the taxon studied, the number of stages within each phase can be variable. In Penaeoidea, there are 5 to 6 naupliar stages, 3 protozoans, 3 mysis and a variable number of decapodids (1-9). On São Paulo coast, only three of the 18 species had their larval development completely described. This project aims to identify larvae in the final stages of Penaeoidea marine shrimp sampled in the plankton integrating morphology and DNA barcoding as identification tool. For this, we intend to compare the data obtained with the sequences in the literature in order to verify the morphological identification; then, obtain DNA fragments (COI and 16S), submit them to the BLAST system in order to check the identity; positioning this sequences in maximum likelihood trees with related species aiming the genetic identification. We expect to contribute to the increase of Dendrobranchiatabiodiversity, to redescribe or describe the final larval stages of marine shrimp and, we want to discover new occurrences for the state of São Paulo. (AU)