Association of bioprinting and 3D printing techniques for the simulation of pulp and bone microenvironments in organ-on-a-chip systems

• Grant number: 23/01623-3
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2023
• End date: September 30, 2025
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Agreement: CNPq
• Principal Investigator: Diana Gabriela Soares dos Passos
• Grantee: Diana Gabriela Soares dos Passos
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
• Associated research grant: 22/05888-9 - Development of strategies for Regenerative Dentistry based on three-dimentional printing technologies, AP.R
• Associated scholarship(s): 24/18054-4 - Evaluation of the printability of polyethylene glycol diacrylate solutions for three-dimensional printing of organ-on-a-chips, BP.IC; 23/09863-3 - Association of bioprinting and 3d printing techniques for the simulation of pulp and bone microenvironments in organ-on-a-chip systems, BP.JD

Abstract

Organ-on-a-chip has emerged as a tool to improve the predictive power of preclinical analyses through an accurate in vitro representation of human physiology, in many cases replacing animal experimentation. This application is achieved through the convergence of the fields of microfabrication, microfluidics and tissue engineering. Thus, this project proposes the development of three organ-on-a-chip platforms with distinct architectures for the evaluation of biomaterials used in bone and pulp regeneration. Therefore, 3D printing and bioprinting techniques will be employed in the confection of the devices, which will be maintained in a perfusion bioreactor to simulate blood flow. Analyses will be performed with 3D cultures of healthy dental pulp stem cells (DPSCs), apical papilla stem cells (SCAPs), human osteoblastic lineage cells (SAOS-2), and human umbilical vein endothelial cells (HUVECs). The chips will be used to investigate cell viability and adhesion and spreading on scaffolds bioprinted with methacrylated gelatin-based bioink functionalized or not with different metal oxides (oxides of, strontium, magnesium and silicon) and produced by mSLA 3D bioprinting. (AU)