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Long-term evaluation of enzymatic activity, bond strength, collagen quality, nanoleakage and surface morphology of restored teeth after cavity preparation performed with high power laser (Er:YAG) or high-speed burs

Grant number: 23/01256-0
Support Opportunities:Regular Research Grants
Duration: September 01, 2023 - August 31, 2025
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Eric Mayer dos Santos
Grantee:Eric Mayer dos Santos
Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated researchers: Lorenzo Breschi ; Patricia Moreira de Freitas Costa e Silva


The present study aims to evaluate in long-term (T0, T6 and T12) the enzymatic activity (zymography and in situ zymography), the bond strength, the collagen quality profile, and the nanoleakage in the adhesive interface of carious and non-carious teeth prepared with diamond burs or Er:YAG laser and restored using different adhesive techniques (self-etching and etching and washing). 120 non-carious human molars and 120 extracted carious human molars will be sectioned to expose the dentin surfaces. The teeth will be assigned to one of the following groups, according to the type of preparation / adhesive technique to which they will be submitted: G1 - Burs/etch-and-rinse; G2 - Burs/self-conditioning; G3 - Er:YAG laser/etch-and-rinse; G4 - Er:YAG/self-etching laser. The bond strength (¼TBS), nanoleakage, Micro-raman, zymography and in situ zymography evaluations will be performed immediately (T0), 6 months after (T6) and twelve months (T12) after aging in artificial saliva. Statistical analysis will be performed using analysis of variance (ANOVA) depending on the evaluation method. (AU)

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