EMU Collections and Infrastructure: acquisition of a robotics system for large-scale, high-performance maintenance and analysis of genetically modified strains of Saccharomyces cerevisiae and other microorganisms

• Grant number: 22/11384-3
• Support Opportunities: Research Infrastructure Program - Collections
• Start date: June 01, 2023
• End date: May 31, 2026
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Luis Eduardo Soares Netto
• Grantee: Luis Eduardo Soares Netto
• Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• City of the host institution: São Paulo
• Associated researchers: Ariel Mariano Silber ; José Renato Rosa Cussiol ; Luciana Elena de Souza Fraga Machado
• Associated scholarship(s): 24/08637-2 - Technical support for a robotic system for large-scale, high-performance maintenance and analysis of genetically modified strains of Saccharomyces cerevisiae, BP.TT

Abstract

The yeast Saccharomyces cerevisiae is the simplest and most widely investigated model organism among eukaryotes. This microorganism can be grown on agar plates or liquid medium; it grows rapidly, and it is easily manipulated genetically and biochemically. Various molecular biology, biochemical and genetic techniques are well established. Furthermore, a repository (SGD = Saccharomyces Genome Database) is public available on the internet (https://www.yeastgenome.org/) with free access of comprehensive and integrated information, stimulating the discovery of functions associated with genes, many of which are conserved in humans. In fact, several relevant contributions have been produced by studies with yeast, for example related to cell cycle regulation; autophagy; transcription; responses to different stresses; mechanisms underlying neurodegeneration among other processes. Some of these contributions have been awarded Nobel Prizes (https://www.yeastgenome.org/blog/a-nobel-prize-for-work-in-yeast-again). Several collections of genetically modified yeasts are maintained and made available free of charge by the Laboratory of Proteins and Redox Biology, coordinated by Dr. Luis Netto (IB-USP). Other collections and strains are also maintained by groups associated with this proposal. It would be important to improve the infrastructure of this resource shared by several Brazilian research groups with the acquisition of the robotic system ROTOR+ and PIXL (https://www.singerinstruments.com/solution/rotorpixl). ROTOR+ and PIXL form a set of equipment that operates with complementary purposes. Colonies selected by PIXL can give rise to libraries that are sorted, replicated, or mated to other strains in ROTOR+. Thus, in addition to being important for carrying out replicas of the collections that we currently have in the laboratories, this robotic systems will make it possible to carry out experiments on a genomic scale such as SGA (Synthetic Genetic Array) and BiFC (bimolecular fluorescence complementation) assays. Furthermore, high throughput screenings should make it possible to determine genotype-phenotype correlations on a genomic scale, through the application of system biology approaches. Another possible outcome of this project is the increased interaction between research groups that will share the use of ROTOR+ and PIXL. Collections of other genetically modified microorganisms may also be maintained and investigated using these robotic systems. (AU)