Effects of preservation methods on the morphology and chemical structure of sub-regions of the human amniotic membrane: study by scanning electron microscopy, immunohistochemistry and Fourier transform infrared spectroscopy.

• Grant number: 23/01140-2
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2023
• End date: October 31, 2025
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Luciana Barros Sant'Anna
• Grantee: Luciana Barros Sant'Anna
• Host Institution: Instituto de Pesquisa e Desenvolvimento (IP&D). Universidade do Vale do Paraíba (UNIVAP). São José dos Campos , SP, Brazil
• Associated researchers: Leandro José Raniero
• Associated scholarship(s): 25/00479-1 - Effects of preservation methods on the morphology and chemical structure of sub-regions of the human amniotic membrane: study by Fourier transform infrared spectroscopy (FTIR)., BP.TT; 24/02350-3 - Effects of preservation methods on the morphology and chemical structure of sub-regions of the human amniotic membrane: study by Fourier transform infrared spectroscopy (FTIR)., BP.TT

Abstract

Amniotic membrane (AM) has attracted attention as a biomaterial for regenerative medicine, due to its biological and mechanical properties, including anti-inflammatory, immunomodulatory, antifibrotic, epithelialization, stability, resistance and flexibility. These properties are attributed to their morphological characteristics and soluble and insoluble bioactive factors produced by their cells and present in the membrane stroma. Recently, differences were verified in the anatomical regions of AM, but it is not yet known whether these regions have different potentials in tissue regeneration, and the effects of different preservation methods on their integrity and morphology. In this context, the objective of this study is to evaluate the morphology, composition and chemical structure of the amniotic membrane in its different anatomical regions, placental amnion and reflected amnion, and in its sides, epithelial and mesenchymal, after different preservation methods. For this, seven full-term human placentas will be obtained at Hospital Santa Casa de São José dos Campos and transported to the laboratory, where, under sterile conditions, the amniotic membranes will be processed and divided into 4 regions (central, peripheral, intermediate and reflected amnion) according to its position related to the umbilical cord. The fragments will be distributed in 2 groups according to the preservation method. Fresca Group: the fragments will be immersed in DMEM medium at room temperature (24ºC) for 24h. Cryopreserved Group: the fragments will be immersed in DMEM/glycerol 1:1 medium at -80ºC for 30 days. After the specific periods of each group, the fragments will be submitted to, scanning electron microscopy, Fourier transform infrared (FT-IR) spectroscopy and immunohistochemistry. (AU)