EVOLUTION OF CATALOGUES OF SATELLITE DNAs IN CHARACIFORMES (Vertebrata: Teleostei): A 100 MILLION YEARS OF HISTORY

• Grant number: 22/13381-1
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2024
• End date: January 31, 2026
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Principal Investigator: Ricardo Utsunomia
• Grantee: Ricardo Utsunomia
• Host Institution: Faculdade de Ciências (FC). Universidade Estadual Paulista (UNESP). Campus de Bauru. Bauru , SP, Brazil
• Associated researchers: Diogo Teruo Hashimoto ; Fábio Porto-Foresti ; Francisco Jesus Ruiz-Ruano Campaña ; GUIGUEN Yann

Abstract

Eukaryotic genomes are mainly composed by a heterogeneous collection of repetitive DNA sequences, in which their functions, mostly, are not exactly known. Within this group, satellite DNAs (satDNAs) are unique tandemly repeated sequences that preferentially occupy heterochromatic regions. Historically, distinct techniques were applied to the characterization of particular satDNAs, like enzymatic restriction of genomes, but only in the last decade, with the spread of next-generation sequencing (NGS) technologies and bioinformatic tools, it became possible to study and characterize representative catalogues of satDNAs (the so-called satellitomes) in several organisms at reduced costs. Recently, the first fish satellitomes were independently disclosed in the Characiformes order, revealing a significative amount of this kind of sequence in the genomes of these species, although a global comparative analysis has not yet been done until now. This, in this study, we aim to characterize and compare additional satellitomes of Characiformes fish (with an estimated origin of 100 million years ago) using short-read sequencing. Besides, chromosome-level genomes of two species will be sequenced and assembled in order to verify if there are specific genomic regions in which conserved satDNAs tend to accumulate. Data obtained here will open the opportunity to investigate, in an unprecedented way, how entire catalogues of satellite DNAs evolve, allowing the identification of evolutionary patterns for this kind of sequence. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DEON, GEIZE APARECIDA; DOS SANTOS, RODRIGO ZENI; SASSI, FRANCISCO DE MENEZES CAVALCANTE; MOREIRA-FILHO, ORLANDO; VICARI, MARCELO RICARDO; PORTO-FORESTI, FABIO; UTSUNOMIA, RICARDO; CIOFFI, MARCELO DE BELLO. The role of satellite DNAs in the chromosomal rearrangements and the evolution of the rare XY₁Y₂ sex system in Harttia (Siluriformes: Loricariidae). JOURNAL OF HEREDITY, v. 115, n. 5, p. 11-pg., 2024-05-17. (23/00955-2, 22/13381-1, 21/14096-6)

DOS SANTOS, RODRIGO ZENI; GOES, CAIO AUGUSTO GOMES; STORNIOLI, JOSE HENRIQUE FORTE; SASSI, FRANCISCO DE MENEZES CAVALCANTE; DE MORAES, RENATA LUIZA ROSA; DERGAM, JORGE ABDALA; PORTO-FORESTI, FABIO; CIOFFI, MARCELO DE BELLO; UTSUNOMIA, RICARDO. Comparative satellite DNA mapping in species of the genus Prochilodus (Teleostei, Characiformes) and its evolutionary implications. GENOME, v. N/A, p. 8-pg., 2024-12-05. (21/14562-7, 23/00955-2, 21/12722-7, 22/13381-1)