Effect of gellan gun sponge hydrogel associated or not to photobiomodulation in full thickness burns

• Grant number: 23/04044-4
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2023
• End date: September 30, 2025
• Field of knowledge: Engineering - Biomedical Engineering
• Principal Investigator: Ana Laura Martins de Andrade
• Grantee: Ana Laura Martins de Andrade
• Host Institution: Universidade Brasil. Campus São Paulo. São Paulo , SP, Brazil
• Associated researchers: Alexandra P. Marques ; Carla Roberta Tim ; Cintia Cristina Santi Martignago ; Lívia Assis Garcia ; Nivaldo Antonio Parizotto

Abstract

The skin is the largest immunologically competent organ in the body and among the most complex injuries that affect it, burns stand out. Full-thickness burns are considered one of the most devastating forms of trauma and, due to the complexity involved in the healing process, the literature has been seeking alternative forms of treatment for this type of injury. Photobiomodulation (FBM) and hydrogels associated with cell therapy are techniques highlighted in the literature with important roles in resolving the repair process at different stages, so it is believed that the association of both treatments can optimize gellan gun-based hydrogels (GG) with mensenchymal stem cells (MSCs) in order to accelerate the repair process and ensure the formation of quality skin tissue. 96 Wister rats will be used, divided into four groups: GC: simulated treatment with FB; GL: treated with FBM (660nm), GH: treated with spongy hydrogel GG-MSCs; GLH: treated with the association of FBM (660nm) and the GG-CTMs spongy hydrogel. The experimental model of full thickness burn will be performed with an aluminum plate with 3 cm in diameter. The treatment of the groups that will use FBM will be performed three times a week, on alternate days for 21 days. The groups that will be treated with hydrogel will receive applications with spongy hydrogel GG-MSCs with the same diameter as the lesion. Photographic monitoring will be carried out after burn induction and in periods of 3, 7, 14 and 21 days after the beginning of the experiment to assess the degree of contraction of the lesion. The animals will be euthanized in four different periods (3rd, 7th, 14th and 21st day after the experimental procedure) with collection of tissues and blood for subsequent histological, immunohistochemical and immunoenzymatic analysis. For statistical analysis, the analysis of variance test, ANOVA two-way will be performed. The significance level will be 5%.This Project aims to start the researches activities of the proponent in the Biomedical Engineering Postgraduate Program from University Brazil (Itaquera Campus) where she is recently full Professor. (AU)