RESISTANCE TRAINING PROGRESSION: EFFECTS ON THE REMODELING OF THE EXTRACELLULAR MATRIX IN HUMANS

• Grant number: 23/04739-2
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2024
• End date: January 31, 2026
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Cleiton Augusto Libardi
• Grantee: Cleiton Augusto Libardi
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Associated researchers: João Guilherme Almeida Bergamasco ; Maíra Camargo Scarpelli ; Michael Roberts
• Associated research grant(s): 24/02221-9 - Are acute and chronic responses in estrogen receptors induced by resistance training associated to muscle hypertrophy in women?, AP.R
• Associated scholarship(s): 24/14207-0 - Resistance training volume in trained individuals: can larger increases promote smaller muscular adaptations?, BP.DR

Abstract

Among the adaptations resulting from resistance training (RT), the increase in cross-sectional area of muscle fibers and, consequently, of the entire muscle (i.e., muscle hypertrophy) may contribute to the prevention or mitigation of obesity, type 2 diabetes, and other comorbidities, thereby preventing premature death. However, several aspects are involved in the prescription of RT, which can affect the regulatory mechanisms of muscle hypertrophy in an acute (i.e., response during and after performing a single RT session) and chronic (i.e., adaptations after performing several sessions) manner, are still not well understood. Our laboratory has successfully investigated, through regular funding from FAPESP (#2013/21218-4; #2017/04299-1; #2018/12150-0), the mechanisms involved in RT-induced muscle hypertrophy. In this project, through an intra-subject randomized controlled trial, we will compare the effects of protocols for the progression of strength training by intensity (progINT) and volume (progVOL) on the cross-sectional area of the vastus lateralis muscle and type I and II fibers, as well as the area occupied by myofibrillar and sarcoplasmic content of these fibers, to investigate the occurrence or absence of sarcoplasmic hypertrophy. Additionally, we will investigate the effects of these protocols on extracellular matrix (ECM) remodeling factors (protein content [MMP-2, MMP-9, MMP-14, TIMP-1, TIMP-2], overall MMP activity, ECM content (collagen area per fiber, collagen-1 and 4 protein expression), the abundance of cells residing and interacting with the ECM (macrophages, FAPs, and satellite cells). Finally, we will also investigate the association between muscle and fiber hypertrophy with markers of extracellular matrix remodeling and whether changes in muscle collagen content and ECM remodeling differ between high and low responders to muscle hypertrophy induced by RT. The rationale that led us to these objectives and our hypotheses can be seen in detail throughout this project. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CHAVES, TALISSON SANTOS; SCARPELLI, MAIRA CAMARGO; BERGAMASCO, JOAO GUILHERME ALMEIDA; DA SILVA, DEIVID GOMES; MEDALHA JUNIOR, RICARDO ALESSANDRO; DIAS, NATHALIA FERNANDA; BITTENCOURT, DIEGO; CARELLO FILHO, PAULO CESAR; ANGLERI, VITOR; NOBREGA, SANMY ROCHA; et al. Effects of Resistance Training Overload Progression Protocols on Strength and Muscle Mass. INTERNATIONAL JOURNAL OF SPORTS MEDICINE, v. N/A, p. 7-pg., 2024-03-12. (23/04739-2)

BERGAMASCO, JOAO G. A.; SCARPELLI, MAIRA C.; GODWIN, JOSHUA S.; MESQUITA, PAULO H. C.; CHAVES, TALISSON S.; DA SILVA, DEIVID G.; BITTENCOURT, DIEGO; DIAS, NATHALIA F.; MEDALHA JUNIOR, RICARDO A.; CARELLO FILHO, PAULO C.; et al. Acute and Chronic Changes in Muscle Androgen Receptor Markers Are Not Associated with Muscle Hypertrophy in Women and Men. MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, v. 56, n. 11, p. 10-pg., 2024-11-01. (23/04739-2)

PLOTKIN, DANIEL L.; MATTINGLY, MADISON L.; ANGLIN, DERICK A.; MICHEL, J. MAX; GODWIN, JOSHUA S.; MCINTOSH, MASON C.; KONTOS, NICHOLAS J.; BERGAMASCO, JOAO G. A.; SCARPELLI, MAIRA C.; ANGLERI, VITOR; et al. Skeletal muscle myosin heavy chain fragmentation as a potential marker of protein degradation in response to resistance training and disuse atrophy. Experimental Physiology, v. 109, n. 10, p. 16-pg., 2024-08-24. (23/04739-2)

BERGAMASCO, JOAO G. A.; SCARPELLI, MAIRA C.; GODWIN, JOSHUA S.; MESQUITA, PAULO H. C.; CHAVES, TALISSON S.; DA SILVA, DEIVID G.; BITTENCOURT, DIEGO; DIAS, NATHALIA F.; MEDALHA JR, RICARDO A.; CARELLO FILHO, PAULO C.; et al. Androgen receptor markers do not differ between nonresponders and responders to resistance training-induced muscle hypertrophy. Journal of Applied Physiology, v. 137, n. 4, p. 9-pg., 2024-10-15. (23/04739-2)

MATTINGLY, MADISON L.; ANGLIN, DERICK A.; RUPLE, BRADLEY A.; SCARPELLI, MAIRA C.; BERGAMASCO, JOAO G.; GODWIN, JOSHUA S.; MOBLEY, CHRISTOPHER B.; FRUGE, ANDREW D.; LIBARDI, CLEITON A.; ROBERTS, MICHAEL D.. Acute and Chronic Resistance Training, Acute Endurance Exercise, nor Physiologically Plausible Lactate In Vitro Affect Skeletal Muscle Lactylation. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 25, n. 22, p. 12-pg., 2024-11-01. (23/04739-2)

BITTENCOURT, DIEGO; DE OLIVEIRA, RAMON MARTINS; DA SILVA, DEIVID GOMES; BERGAMASCO, JOAO GUILHERME ALMEIDA; CESAR, MARCELO DE CASTRO; JACOMASSI, DANIELA GODOI; DE CAMARGO, JULIO BENVENUTTI BUENO; KINGSLEY, J. DEREK; LIBARDI, CLEITON AUGUSTO. Effects of individualized resistance training prescription with heart rate variability on muscle strength, muscle size and functional performance in older women. FRONTIERS IN PHYSIOLOGY, v. 15, p. 9-pg., 2024-12-17. (23/04739-2, 16/25401-6)

SCARPELLI, MAIRA C.; BERGAMASCO, JOAO G. A.; GODWIN, JOSHUA S.; MESQUITA, PAULO H. C.; CHAVES, TALISSON S.; SILVA, DEIVID G.; BITTENCOURT, DIEGO; DIAS, NATHALIA F.; MEDALHA JUNIOR, RICARDO A.; CARELLO FILHO, PAULO C.; et al. Resistance training-induced changes in muscle proteolysis and extracellular matrix remodeling biomarkers in the untrained and trained states (Apr, 10.1007/s00421-024-05484-5, 2024). EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY, v. N/A, p. 1-pg., 2024-06-15. (23/04739-2)