Adaptations to the lengths of the sarcomeres in the region of the myotendinous junction of the plantar muscle associated to physical exercise

• Grant number: 17/21977-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): March 01, 2018
• Effective date (End): December 31, 2018
• Field of knowledge: Biological Sciences - Morphology - Anatomy
• Principal Investigator: Adriano Polican Ciena
• Grantee: Jurandyr Pimentel Neto
• Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil

Abstract

The muscular tissue presents plasticity consequent of the physical activity in adults it has the capacity of regeneration integrates after certain types of lesions. This basic contractile unit is called sarcomere highly structured and ordered. The myotendinous junction (MTJ) consists of a highly specialized region where the connection between the sarcoplasmic membranes and the collagen fibers of the extracellular matrix occurs this region represents the largest area of force transfer of skeletal muscle besides being the most susceptible point to sports injuries. Physical exercise is recommended for each muscle group of 2x to 3x / week, this type of training results in increased muscle strength associated with improved motor performance as well as gains in physical endurance and positive metabolic adaptations. The present study will describe the metabolic, biochemical and morphofunctional analyzes of the plantar muscle of adult Wistar rats submitted to aerobic exercise and resistance exercise. In addition to the hypothesis of possible differentiation in sarcomere lengths. In this way thirty adult Wistar rats (90 days) randomly divided into three groups will be used: Control Group (C): not submitted to physical exercise protocol; Aerobic Training Group (AT): submitted to the protocol without overload; Resisted Training Group (RT): submitted to protocol with overload. For the trainings will be used the vertical ladder where the animals will be adapted and trained they will perform a total of 9 climbs in the AT Group without adding overload and in the RT Group of 4 to 9 climbs with addition of overloads the animals will have 3 attempts by climbing the AT Group will have 1 minute rest between the sets and the RT Group 2 minutes the protocol will total in 24 sessions 3x/week. A muscle power test (amount of movements x overhead) will be performed pre and post protocol. For functional analysis will be recorded the time by climbing and the amount of movements performed by the animal. For the analysis of light microscopy, Hematoxylin-Eosin and Picro-Sirius techniques will be used and for histochemical technique of myofibrillar ATPase for morphometric analysis of the sarcomeres lengths will be performed for ultrastructural analysis (transmission electron microscopy).Keywords: Sarcomeres in series , Myotendinous Junction; Physical Exercise; Muscle Hypertrophy (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ROCHA, LARA CAETANO; PIMENTEL NETO, JURANDYR; DE SANT'ANA, JOSSEI SOARES; JACOB, CAROLINA DOS SANTOS; BARBOSA, GABRIELA KLEIN; KRAUSE NETO, WALTER; WATANABE, II-SEI; CIENA, ADRIANO POLICAN. Repercussions on sarcomeres of the myotendinous junction and the myofibrillar type adaptations in response to different trainings on vertical ladder. MICROSCOPY RESEARCH AND TECHNIQUE, v. 83, n. 10, JUN 2020. (17/21977-3, 18/09199-8)

NETO, JURANDYR PIMENTEL; ROCHA, LARA CAETANO; JACOB, CAROLINA DOS SANTOS; BARBOSA, GABRIELA KLEIN; CIENA, ADRIANO POLICAN. ostsynaptic cleft density changes with combined exercise protocols in an experimental model of muscular hypertroph. EUROPEAN JOURNAL OF HISTOCHEMISTRY, v. 65, n. 1, SI, 2021. (17/12525-1, 17/21977-3)

NETO, JURANDYR PIMENTEL; ROCHA, LARA CAETANO; BARBOSA, GABRIELA KLEIN; JACOB, CAROLINA DOS SANTOS; NETO, WALTER KRAUSE; WATANABE, II-SEI; CIENA, ADRIANO POLICAN. Myotendinous junction adaptations to ladder-based resistance training: identification of a new telocyte niche. SCIENTIFIC REPORTS, v. 10, n. 1, AUG 24 2020. (17/12525-1, 17/21977-3, 18/09199-8)