Role of the P11-4 peptide in the tissue mineralization using a 3D bioprinted dentin model and human dental pulp stem cells.

• Grant number: 23/10291-4
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2024
• End date: January 31, 2026
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Fábio Dupart Nascimento
• Grantee: Fábio Dupart Nascimento
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated researchers: Marimélia Aparecida Porcionatto ; Regina Maria Puppin Rontani

Abstract

Worldwide, dental caries is the most common chronic disease, with a significant impact on public health expenditures and the quality of life of individuals. Epidemiological data indicate the situation is critical in several countries, affecting 60-90% of school-age children. Despite the advances that have occurred in recent years regarding materials and techniques used for oral hygiene, demineralization, and cavitation of mineralized dental tissues (enamel and dentin) is still a public health problem. Several biomimetic strategies have been developed to achieve collagen mineralization, inspired by the behavior of the protein matrix in biomineralization processes. In this sense, recent studies by our group have demonstrated the mineralizing capacity of P11-4, a self-organizing synthetic peptide, derived from DMP-1, with an amphiphilic character that can form ² sheets when exposed to physiological stimuli. Its cellular activity is related to cell adhesion and differentiation and improved repair of dental tissues. ¬The present proposal aims to produce, through 3D bioprinting, a mineralizable and biologically functional dentin matrix from DPSCs and, subsequently, to evaluate the mechanisms by which P11-4 can modulate the dentin neoformation process. To this end, state-of-the-art technologies will be used to prepare and characterize the bioprinted dentin matrix, with high potential for developing an innovative product, as well as the subsequent evaluation of the molecular mechanisms of action of P11-4 in guided dentin mineralization. In addition to generating more knowledge about the functional role of the P11-4 peptide as a scaffold in dentin mineralization, this study also proposes to build a new product with significant market appeal, sustainability, and potential clinical use. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALLENDE, JAVIER BASUALDO; NASCIMENTO, FABIO DUPART; CHIARI, MARINA DAMASCENO E SOUZA; ALIAGA-GALVEZ, ROMINA; NAUPARI-VILLASANTE, ROMINA; MIRANDA, CRISTIAN BERSEZIO; PARDO-DIAZ, CAROLINA; GUTIERREZ, MARIO FELIPE; COVARRUBIAS, CRISTIAN; LOGUERCIO, ALESSANDRO D.; et al. Evaluation of adhesive properties and enzymatic activity at the hybrid layer of a simplified adhesive loaded with 0.2 % Cu and 5 % ZnO nanoparticles: A Randomized Clinical Trial and ex vivo analysis. Journal of Dentistry, v. 149, p. 9-pg., 2024-08-28. (23/10291-4)