Test of orthopedic implant enriched with graphene for application in surgical medicine.

• Grant number: 23/09675-2
• Support Opportunities: Research Grants - Innovative Research in Small Business - PIPE
• Start date: September 01, 2024
• End date: May 31, 2025
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Mateus Saito
• Grantee: Mateus Saito
• Company: MS MEDICAL SERVICES LTDA
• CNAE: Pesquisa e desenvolvimento experimental em ciências físicas e naturais
• Associated researchers: Ines Pereyra ; Marcelo Nelson Páez Carreño ; Tiago Lazzaretti Fernandes

Abstract

Fractures are the most common traumatic injuries to large organs in humans, requiring, in some cases, surgical procedures. The most common complications of fractures from both bone injuries and osteochondritis dissecans (OCD) are: delayed union, pseudarthrosis, infection or implant failure. Prevention of complications occurs through the surgeon's understanding and respect for the biology of the bone, periosteum and soft tissues, which will avoid failures after open reduction and fixation, whatever it may be, and also with the use of various equipment, possible frameworks, artifacts, instruments and implants in the various existing procedures. The project team has already managed to both synthesize and characterize graphene with derivatives of interest, and enrich orthopedic implants used in surgeries with graphene to provide properties of interest, for example, osteoimmunomodulatory, improvements in the regeneration of musculoskeletal tissue and biocompatibility. To contextualize the degree of innovation of the proposal, in a search on Pubmed using the words surgical and orthopedic implant, 35,266 results are presented, but adding the keyword graphene, only 22 results are presented. The project team has already carried out the synthesis of Graphene, enrichment of surgical cement of the acrylic bone cement variety - acrylic bone cements (ABCs), as well as enrichment of orthopedic implants, including the characterization of the material including infrared spectroscopy with Fourier transform (FTIR); scanning electron microscopy (SEM), nuclear magnetic resonance (1H NMR), Raman mapping and spectroscopy, thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), with promising results. In view of this, it is proposed in this project to carry out tests specifically on orthopedic implant enriched with ABNT NBR ISO 5833:2004 compression resistance graphene, dynamic mechanical analysis (DMA), hydrolytic degradation, cell viability, alkaline phosphatase activity (ALP) with human osteoblasts (HOb), antibacterial action against gram-negative bacteria Escherichia Coli and finally the in Vivo osseointegration of the designed compounds. If positive results are obtained, it is intended to submit a proposal for phase 2 and develop the product for scalability. (AU)