Physico-mechanical, antibacterial and cytotoxicity properties of a 3D-printed resin composite containing Biosilicate glass-ceramic

• Grant number: 24/04874-0
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2024
• End date: November 30, 2027
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Vanessa Cavalli Gobbo
• Grantee: Vanessa Cavalli Gobbo
• Principal researcher abroad: Mutlu Özcan
• Institution abroad: University of Zurich (UZH), Switzerland
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
• Associated researchers: Marcelo Giannini
• Associated scholarship(s): 24/23557-5 - Development of experimental 3D printed composite resin containing Biosilicate: mechanical, antibacterial, and cytotoxic properties., BP.DR

Abstract

The prompt use of additive technology in Dentistry has granted the development of 3D-printing resin composite for definite restorations (3D-RC). However, because of the clinical necessity for polymer-based materials with enhancing bioactive and biomimetic properties, this study aims to develop an experimental 3D-RC incorporated with Biosilicate (3D-BioS) and evaluate the physical-mechanical, antibacterial and cytotoxicity properties of the material. In Phase 1, BioS (0, 5, 10, 15 and 20%wt) will be synthetized, added to 3D-RC and characterized. In Phase 2, the physical-mechanical properties (flexural strength, flexural modulus) will be tested, and the best 3D-BioS concentrations will be further evaluated by contact angle, microhardness, surface roughness average, microstructure of the 3D-BioS, and sorption and solubility, before (T0) and after (TF) simulated aging (thermal cycling and simulated mechanical toothbrushing). In Phase 3, S. mutans monospecies biofilm formation and the 3D distribution of cellular and extracellular components of the biofilm formed on the 3D-BioS before and after simulated aging will be tested, and in Phase 4, 3D-BioS cytotoxicity in human gingival fibroblasts will be evaluated, before and after simulated aging. A commercial 3D-RC will be used as the control group for all the research phases. Statistical analysis will be conducted under a significance level of 5%. (AU)