Evaluation of behavior of Salmonella Gallinarum, Salmonella Pullorum, and Salmonella Enteritidis strains during chicken-primary-macrophage infection

• Grant number: 23/07046-8
• Support Opportunities: Research Grants - Young Investigators Grants
• Start date: April 01, 2025
• End date: March 31, 2030
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Mauro de Mesquita Souza Saraiva
• Grantee: Mauro de Mesquita Souza Saraiva
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Associated researchers: Angelo Berchieri Junior
• Associated scholarship(s): 25/10691-8 - Uptake and survival assays of Salmonella Gallinarum, Salmonella Pullorum and Salmonella Enteritidis strains during the infection of macrophage-like chicken cells type HD11., BP.IC; 25/05458-2 - Primary macrophage infection assays of chickens by strains of Salmonella Gallinarum, Salmonella Pullorum and Salmonella Enteritidis: an evaluation of bacterial gene expression during intramacrophage infection, BP.TT; 25/05413-9 - Evaluation of behavior of Salmonella Gallinarum, Salmonella Pullorum, and Salmonella Enteritidis strains during chicken-primary-macrophage infection., BP.JP

Abstract

Among the salmonellosis diseases of public health importance, S. Enteritidis (SE), which is also relevant for the poultry industry, even as S. Gallinarum biovars Gallinarum (SG) and Pullorum (SP), is responsible for the development of fowl typhoid and Pullorum disease, respectively. During the pathogenesis, innate immunity plays an essential role, since the bacteria, through virulence mechanisms, can establish and multiply inside macrophages, spreading to other organs such as the spleen and liver. Thus, the present study aims to perform the infection of chicken primary macrophages and avian macrophage-like cells (immortalized HD11 cell line), comparing the ability of capture and survival between SE, SG, and SP strains and the response between both cell lines. Furthermore, it is necessary to understand which virulence mechanisms are expressed during the cellular infection process by performing RNA sequencing, allowing the analysis of the set of transcripts generated, unlike hybridization techniques, that work with specific targets. To perform the sequencing, we aim to deplete the ribosomal RNA of both species, and this represents a challenge since the available kits are for joint depletion focused on mammals. Finally, to obtain a holistic view of the behavior of bacteria during infection of chicken primary macrophages, and consequently a better understanding of the pathogen-host interaction, analysis of the set of metabolites (metabolomics) generated during the process will be performed, providing further information on the metabolic reprogramming that these cells undergo. (AU)