Pathogenicity of Salmonella spp. with deletions in the genes phoPQ, fliD e clpP in comercial poultry

• Grant number: 16/10369-0
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2016
• End date: February 28, 2019
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Angelo Berchieri Junior
• Grantee: Angelo Berchieri Junior
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Associated researchers: Oliveiro Caetano de Freitas Neto ; Rafael Antonio Casarin Penha Filho

Abstract

The aviculture is an important segment of animal production. The intensive system favours the emergence of various infections, like salmonellosis. The fowl typhoid, caused by Salmonella Gallinarum biovar Gallinarum (SG) causes high mortality rate on the flock, whereas Salmonella Enteritidis (SE) and Salmonella Typhimurium (ST) outcomes in disorders to the poultry production because may induce disease in poultry and, through food poultry origin, to provokehuman foodborne disease. Soto take systemic infection, SG has to invade the host, to evade the antimicrobial activity of the macrophages and to multiply inside the phagossomes, being indispensable the activation of the pleiotropic two-component regulatory system PhoP/PhoQ during the process. On the other hand, the previous recognition of flagellin of paratyphoid salmonellosis by the intestinal TLR-5 receptors may turns the systemic infection into mild. With the purpose to evaluate the infection of mutant strains SG-phoPQ, SE-fliD-clpP and ST-fliD-clpP, two assays will be performed in susceptible poultry. The first one aims to survey the effect caused by the deletion of phoP and phoQ (SG-phoPQ) genes on SG pathogenicity and, in case of the strain is attenuated, itsabilityto induce protection to poultry against fowl typhoid and fowl paratyphoid caused by SE will be evaluated. The assay will be based on the evaluation of intestinal colonisation, systemic infection and the characterization of the activity of macrophages and lymphocytes in poultry inoculated with strains SE-fliD-clpP and ST-fliD-clpP will be performed. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BARBOSA, FERNANDA DE OLIVEIRA; DE FREITAS NETO, OLIVEIRO CAETANO; RODRIGUES ALVES, LUCAS BOCCHINI; BENEVIDES, VALDINETE PEREIRA; SECUNDO DE SOUZA, ANDREI ITAJAHY; RUBIO, MARCELA DA SILVA; DE ALMEIDA, ADRIANA MARIA; SARAIVA, MAURO MESQUITA; BRUNO DE OLIVEIRA, CELSO JOSE; OLSEN, JOHN ELMERDAHL; et al. Immunological and bacteriological shifts associated with a flagellin-hyperproducing Salmonella Enteritidis mutant in chickens. Brazilian Journal of Microbiology, v. 52, n. 1, SI, NOV 2020. (18/04883-8, 16/10369-0)

VP BENEVIDES; MS RUBIO; LBR ALVES; FO BARBOSA; AIS SOUZA; AM ALMEIDA; MRT CASAS; EAL GUASTALLI; NM SOARES; A BERCHIERI JR. Antimicrobial Resistance in Salmonella Serovars Isolated From an Egg-Producing Region in Brazil. Brazilian Journal of Poultry Science, v. 22, n. 2, 2020-10-07. (16/10369-0, 17/25743-7)

RODRIGUES ALVES, LUCAS BOCCHINI; DE FREITAS NETO, OLIVEIRO CAETANO; ALVES BATISTA, DIEGO FELIPE; BARBOS, FERNANDA DE OLIVEIRA; RUBIO, MARCELA DA SILVA; SECUNDO DE SOUZA, ANDREI ITAJAHY; DE ALMEIDA, ADRIANA MARIA; BARROW, PAUL ANDREW; BERCHIERI JUNIOR, ANGELO. Inactivation of phoPQ genes attenuates Salmonella Gallinarum biovar Gallinarum to susceptible chickens. Brazilian Journal of Microbiology, v. 49, n. 3, p. 601-606, JUL-SEP 2018. (16/10369-0)

BARBOSA, FERNANDA DE OLIVEIRA; DE FREITAS NETO, OLIVEIRO CAETANO; RODRIGUES ALVES, LUCAS BOCCHINI; BENEVIDES, VALDINETE PEREIRA; SECUNDO DE SOUZA, ANDREI ITAJAHY; RUBIO, MARCELA DA SILVA; DE ALMEIDA, ADRIANA MARIA; SARAIVA, MAURO MESQUITA; BRUNO DE OLIVEIRA, CELSO JOSE; OLSEN, JOHN ELMERDAHL; et al. Immunological and bacteriological shifts associated with a flagellin-hyperproducing Salmonella Enteritidis mutant in chickens. Brazilian Journal of Microbiology, v. 52, n. 1, p. 11-pg., 2020-11-04. (16/10369-0, 18/04883-8)