Evaluation of avian infection (Gallus gallus domesticus) by Salmonella enteritidis, Salmonella typhimurium and Salmonella heidelberg containing deletion of the ttrA and pduA genes

• Grant number: 18/03189-0
• Support Opportunities: Research Projects - Thematic Grants
• Duration: August 01, 2018 - January 31, 2024
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Angelo Berchieri Junior
• Grantee: Angelo Berchieri Junior
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Associated scholarship(s): 22/15869-1 - Evaluation of the cellular immune response of mutant strains of S. Enteritidis (SEttrApduA) and S. Typhimurium (STMttrApduA) in birds (Gallus gallus domesticus), BP.TT; 22/15870-0 - Evaluation of mice infection (Mus musculus) by Salmonella Typhimurium in absence of ttrA and pduA genes, BP.IC; 21/00717-9 - Evaluation of intestinal colonization and systemic infection by SE (NalrSpecr), STM (NalrSpecr) and SH (NalrSpecr) previously inoculated by the mutant strains SE ttrA pduA, STM ttrA pduA and SH ttrA pduA, BP.IC; + associated scholarships 20/06076-2 - Evaluation of avian infection (Gallus gallus domesticus) by Salmonella Enteritidis, Salmonella Typhimurium and Salmonella Heidelberg containing deletion of the ttrA and pduA genes, BP.PD; 19/23298-1 - Evaluation of avian infection (Gallus gallus domesticus) by Salmonella Enteritidis, Salmonella Typhimurium and Salmonella Heidelberg containing deletion of the ttrA and pduA genes, BP.TT; 19/01809-4 - Evaluation of intestinal colonization and systemic infection of mutant strains of S. Enteritidis (SE”ttrA”pduA), S. Typhimurium (STM”ttrA”pduA) e S. Heidelberg (SH”ttrA”pduA) in chicken (Gallus gallus domesticus), BP.IC; 18/21301-2 - Evaluation of avian infection (Gallus gallus domesticus) by Salmonella enteritidis, Salmonella typhimurium and Salmonella heidelberg containing deletion of the ttrA and pduA genet, BP.PD; 18/19438-0 - Fecal excretion of mutant strains of S. Enteritidis (SE”ttrA”pduA), S. Typhimurium (STM”ttrA”pduA) and S. Heidelberg (SH”ttrA”pduA) in chicken (Gallus gallus domesticus), BP.TT - associated scholarships

Abstract

Assays done in mammals has shown that enteric salmonellas are able to use the inflammatory process provoked by them as a source of energy to survive and multiply inside the gut. This process is associated with the use of tetrathionate (ttr) as a by-product of the host inflammatory gut response. After the production of tetrathionate it becomes possible to use propanediol (pdu) as an energy source. Therefore, the intestinal inflammatory response of the host promotes efficient bacterial multiplication in the intestinal lumen, with consequent colonization and fecal-oral transmission. Poultry products have been associated with human foodborne diseases. Most of them caused by Salmonella enteritidis (SE) and Salmonella typhimurium (ST). In the last years, the European Community has rejected several poultry products from Brazil because they were contaminated by Salmonella heidelberg (SH). In view of the importance of poultry foods in foodborne salmonellosis we will investigate the relevance of genes related to tetrathionate and ethanolamine metabolism to the intestinal colonization of Salmonella in chickens. It is proposed the construction of mutant strains of SE, ST and SH containing deletions in the genes ttrA and pduA, to assess their behavior during the infection. The knowledge acquired may help to better understand the relationship between the bacteria and the host, contributing to a better understanding of bacterial behaviour and the search for alternatives to inhibit intestinal survival and colonization. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SARAIVA, M. M. S.; RODRIGUES ALVES, L. B.; MONTE, D. F. M.; FERREIRA, T. S.; BENEVIDES, V. P.; BARBOSA, F. O.; FREITAS NETO, O. C.; ALMEIDA, A. M.; BARROW, P. A.; BERCHIERI JUNIOR, A.. Deciphering the role of ttrA and pduA genes for Salmonella enterica serovars in a chicken infection model. AVIAN PATHOLOGY, v. 50, n. 3, p. 257-268, MAY 4 2021. (20/06076-2, 18/21301-2, 18/03189-0)

CABRERA, JULIA M.; SARAIVA, MAURO M. S.; ALVES, LUCAS RODRIGUES B.; MONTE, DANIEL F. M.; VASCONCELOS, ROSEMERI O.; FREITAS NETO, OLIVEIRO C.; BERCHIERI JUNIOR, ANGELO. Salmonella enterica serovars in absence of ttrA and pduA genes enhance the cell immune response during chick infections. SCIENTIFIC REPORTS, v. 13, n. 1, p. 9-pg., 2023-01-11. (18/03189-0, 20/06076-2)

TS FERREIRA; R RAVETTI; MS RUBIO; LBR ALVES; MMS SARAIVA; VP BENEVIDES; TS LIMA; BN LIMA; AM ALMEIDA; A BERCHIERI JR. Inclusion of Organic Acids in the Drinking Water and Feed for the Control of Salmonella Heidelberg in Broilers. Brazilian Journal of Poultry Science, v. 24, n. 2, 2022-04-22. (19/01809-4, 18/03189-0)

SARAIVA, M. M. S.; SILVA, N. M., V; FERREIRA, V. A.; MOREIRA FILHO, A. L. B.; GIVISIEZ, P. E. N.; NETO, O. C. FREITAS; JUNIOR, A. BERCHIERI; GEBREYES, W. A.; OLIVEIRA, C. J. B.. Residual concentrations of antimicrobial growth promoters in poultry litter favour plasmid conjugation among Escherichia coli. Letters in Applied Microbiology, v. 74, n. 5, p. 8-pg., 2022-02-21. (18/03189-0, 18/21301-2)

RUBIO, MARCELA DA SILVA; RODRIGUES ALVES, LUCAS BOCCHINI; VIANA, GUILHERME DE BRITO; BENEVIDES, VALDINETE PEREIRA; SPINA DE LIMA, TULIO; SANTIAGO FERREIRA, TAISA; ALMEIDA, ADRIANA MARIA DE; BARROW, PAUL ANDREW; BERCHIERI JUNIOR, ANGELO. Heat stress impairs egg production in commercial laying hens infected by fowl typhoid. AVIAN PATHOLOGY, v. 50, n. 2, JAN 2021. (19/23634-1, 18/03189-0)