Evaluation of avian infection (Gallus gallus domesticus) by Salmonella enteritidis, Salmonella typhimurium and Salmonella heidelberg containing deletion of the ttrA and pduA genet

• Grant number: 18/21301-2
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Effective date (Start): December 01, 2018
• Effective date (End): November 30, 2023
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Angelo Berchieri Junior
• Grantee: Mauro de Mesquita Souza Saraiva
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Associated research grant: 18/03189-0 - Evaluation of avian infection (Gallus gallus domesticus) by Salmonella enteritidis, Salmonella typhimurium and Salmonella heidelberg containing deletion of the ttrA and pduA genes, AP.TEM
• Associated scholarship(s): 20/07541-0 - Influence of Salmonella enteritidis and Salmonella typhimurium under gut microbiome from broilers, after deletion of pduA and ttrA genes: a genomic approach, BE.EP.PD

Abstract

Assays done in mammals has shown that enteric salmonellas are able to use the inflammatory process provoked by them as a source of energy to survive and multiply inside the gut. This process is associated with the use of tetrathionate (ttr) as a by-product of the host inflammatory gut response. After the production of tetrathionate it becomes possible to use propanediol (pdu) as an energy source. Therefore, the intestinal inflammatory response of the host promotes efficient bacterial multiplication in the intestinal lumen, with consequent colonization and fecal-oral transmission. Poultry products have been associated with human foodborne diseases. Most of them caused by Salmonella enteritidis (SE) and Salmonella typhimurium (ST). In the last years, the European Community has rejected several poultry products from Brazil because they were contaminated by Salmonella heidelberg (SH). In view of the importance of poultry foods in foodborne salmonellosis we will investigate the relevance of genes related to tetrathionate and ethanolamine metabolism to the intestinal colonization of Salmonella in chickens. It is proposed the construction of mutant strains of SE, ST and SH containing deletions in the genes ttrA and pduA, to assess their behaviour during the infection. The knowledge acquired may help to better understand the relationship between the bacteria and the host, contributing to a better understanding of bacterial behaviour and the search for alternatives to inhibit intestinal survival and colonization.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SARAIVA, M. M. S.; RODRIGUES ALVES, L. B.; MONTE, D. F. M.; FERREIRA, T. S.; BENEVIDES, V. P.; BARBOSA, F. O.; FREITAS NETO, O. C.; ALMEIDA, A. M.; BARROW, P. A.; BERCHIERI JUNIOR, A.. Deciphering the role of ttrA and pduA genes for Salmonella enterica serovars in a chicken infection model. AVIAN PATHOLOGY, v. 50, n. 3, p. 257-268, MAY 4 2021. (20/06076-2, 18/21301-2, 18/03189-0)

SARAIVA, M. M. S.; SILVA, N. M., V; FERREIRA, V. A.; MOREIRA FILHO, A. L. B.; GIVISIEZ, P. E. N.; NETO, O. C. FREITAS; JUNIOR, A. BERCHIERI; GEBREYES, W. A.; OLIVEIRA, C. J. B.. Residual concentrations of antimicrobial growth promoters in poultry litter favour plasmid conjugation among Escherichia coli. Letters in Applied Microbiology, v. 74, n. 5, p. 8-pg., 2022-02-21. (18/03189-0, 18/21301-2)

SOUSA SARAIVA, MAURO DE MESQUITA; BENEVIDES, VALDINETE PEREIRA; VIEIRA DA SILVA, NUBIA MICHELLE; VARANI, ALESSANDRO DE MELLO; DE FREITAS NETO, OLIVEIRO CAETANO; BERCHIERI, ANGELO; DELGADO-SUAREZ, ENRIQUE JESUS; DE LIMA ROCHA, ALAN DOUGLAS; EGUALE, TADESSE; MUNYALO, JANET AGNES; et al. Genomic and Evolutionary Analysis of Salmonella enterica Serovar Kentucky Sequence Type 198 Isolated From Livestock In East Africa. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 12, p. 12-pg., 2022-06-20. (18/21301-2)

SOUZA SARAIVA, MAURO DE MESQUITA; LIM, KELVIN; MARINHO DO MONTE, DANIEL FARIAS; NAVES GIVISIEZ, PATRICIA EMILIA; RODRIGUES ALVES, LUCAS BOCCHINI; DE FREITAS NETO, OLIVEIRO CAETANO; KARIUKI, SAMUEL; BERCHIERI JUNIOR, ANGELO; BRUNO DE OLIVEIRA, CELSO JOSE; GEBREYES, WONDWOSSEN ABEBE. ntimicrobial resistance in the globalized food chain: a One Health perspective applied to the poultry industr. Brazilian Journal of Microbiology, v. 53, n. 1, NOV 2021. (20/06076-2, 18/21301-2)