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Modern techniques in mass spectrometry and their applications in chemistry and biochemistry

Grant number:00/11176-2
Support Opportunities:Research Projects - Thematic Grants
Start date: February 01, 2001
End date: October 31, 2005
Field of knowledge:Physical Sciences and Mathematics - Chemistry
Principal Investigator:Marcos Nogueira Eberlin
Grantee:Marcos Nogueira Eberlin
Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
City of the host institution:Campinas
Associated scholarship(s):01/05888-2 - Using ESI-MS technique (electrospray ionization mass spectrometry) for the determination and identification of DNA damage, BP.DR

Abstract

Modern techniques in mass spectrometry (MS) will be applied in research projects associated with a variety of chemistry and biochemistry fields: (i) high resolution mass spectrometry and high energy tandem MS/MS, in detailed studies of dissociation mechanisms of different classes of organic compounds via EI e CI mass spectra, accurate mass measurements and MS/MS; (ii) sequential pentaquadrupole mass spectrometry MS/MS e MS/MS/MS, in gas-phase ion/molecule reaction studies and their analytical and synthetic applications, and in the measurement of thermochemical properties. Reactivity and mechanism studies of ions and ionic reaction intermediates; (iii) membrane introduction mass spectrometry (MIMS) and its derived techniques CT(Cryotrap)-MIMS, HS(headspace)-MIMS e T&R(trap&release)-MIMS, in the monitoring of volatile (VOCs) and semi-volatile organic compounds (SVOCs) in water, soil, solid matrixes, oil, viscous liquids, foods, beverages, and biological fluids (plasma and urine); (iv) tandem mass spectrometry MS/MS (QQTOF) with Electrospray ionization (ESI-MS), in a variety of applications: chiral recognition, studies of supramolecular structures, gas-phase reactivity of organometalics, metal-activated reactivity of molecules, monitoring in aqueous media of transition metal-catalyzed coupling reactions, accurate mass measurements of thermolabile compounds, detection of intermediates in catalytic processes, monitoring of dye degradation in water, DNA analysis, ion/molecule reaction studies in the gas phase with ESI-generated ions and their analytical and synthetic applications, and in the measurement of thermochemical properties, analysis of chemicals of medical and pharmaceutical interest, and of their metabolites in biological fluids. The research projects will be performed using the mass spectrometers currently available at the UNICAMP MS laboratory: High resolution MS/MS Autospec EBE, pentaquadrupo, MIMS-Q and GCIMS, and an additional and versatile tandem MS/MS mass spectrometer, ESI-QTof, that will permit innovative application of MS techniques in a variety of chemical and biochemical fields. (AU)

Articles published in Agência FAPESP Newsletter about the research grant:
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VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications (5)
(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)
RIOLI, VANESSA; GOZZO, FABIO C.; HEIMANN, ANDREA S.; LINARDI, ALESSANDRA; KRIEGER, JOSÉ E.; SHIDA, CLÁUDIO S.; ALMEIDA, PAULO C.; HYSLOP, STEPHEN; EBERLIN, MARCOS N.; FERRO, EMER S.. Novel natural peptide substrates for endopeptidase 24.15, neurolysin and angiotensin-converting enzyme. Journal of Biological Chemistry, v. 278, n. 10, p. 8547-8555, . (96/01451-9, 00/11176-2)
CERCHIARO‚ G.; SABOYA‚ P.L.; DA COSTA FERREIRA‚ A.M.; TOMAZELA‚ D.M.; EBERLIN‚ M.N.. Keto-enolic equilibria of an isatin-schiff base copper (II) complex and its reactivity toward carbohydrate oxidation. TRANSITION METAL CHEMISTRY, v. 29, n. 5, p. 495-504, . (00/11176-2, 01/07309-0)
HEIMANN‚ AS; FAVARATO‚ MH; GOZZO‚ FC; RIOLI‚ V.; CARRENO‚ FR; EBERLIN‚ MN; FERRO‚ ES; KREGE‚ JH; KRIEGER‚ JE. ACE gene titration in mice uncovers a new mechanism for ACE on the control of body weight. Physiological Genomics, v. 20, n. 2, p. 173-182, . (00/11176-2, 01/00009-0)
ALVES‚ W.A.; CERCHIARO‚ G.; PADUAN-FILHO‚ A.; TOMAZELA‚ D.M.; EBERLIN‚ M.N.; DA COSTA FERREIRA‚ A.M.. Infinite zig-zag and cyclic-tetranuclear isomeric imidazolate-bridged polynuclear copper (II) complexes: Magnetic properties‚ catalytic activity and electrospray mass and tandem mass spectrometry characterization. Inorganica Chimica Acta, v. 358, n. 13, p. 3581-3591, . (00/11176-2)
SAWAYA‚ A.C.H.F.; TOMAZELA‚ D.M.; CUNHA‚ I.B.S.; BANKOVA‚ V.S.; MARCUCCI‚ M.C.; CUSTODIO‚ A.R.; EBERLIN‚ M.N.. Electrospray ionization mass spectrometry fingerprinting of propolis. ANALYST, v. 129, n. 8, p. 739-744, . (00/11176-2)

Filed patent(s) as a result of this research project

PROCESSO PARA IDENTIFICAÇÃO DE GASOLINAS ADULTERADAS POR SOLVENTES DE DETECÇÃO DE MARCADORES QUÍMICOS POR ESPECTROMETRIA DE MASSAS COM IONIZAÇÃO À PRESSÃO ATMOSFÉRICA PI 0503345-4 - Universidade Estadual de Campinas Unicamp . Renato Haddad; Rodinei Augusti; Marcos Nogueira Eberlin; Daniella Vasconcellos Augusti - January 2005, 01