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Phage display and immune system: identification of molecular targets with diagnostic and therapeutic properties in immunological disorders

Grant number: 04/02721-8
Support type:Research Projects - Thematic Grants
Duration: March 01, 2005 - October 31, 2008
Field of knowledge:Biological Sciences - Immunology
Principal Investigator:Jorge Elias Kalil Filho
Grantee:Jorge Elias Kalil Filho
Home Institution: Fundação Zerbini. São Paulo , SP, Brazil
Co-Principal Investigators:Luisa Lina Villa
Associated grant(s):06/54249-6 - Identification of molecular markers of atherosclerotic vessels, AR.EXT

Abstract

Peptide phage display is a powerful tool that utilizes bacteriophages to screen cells, tissues or organs in search of receptor-pair ligands. Phage display particles are like biological nanoprobes, capable of interacting with the target though small peptides, genetically engineered to be expressed on the virus outer coat. Since the peptide sequence information is coded by bacteriophage genome, a single virus particle bound to a cell or tissue sample can be recovered by bacterial infection, amplified and the peptide identified. Few techniques allow studying molecular interactions of single molecules or cell without previous knowledge of the target. The aim of this project is to establish a web of knowledge and think tank in phage display technology in Brazil. The technique will be employed at full capacity, in different approaches but the main theme will be the immune system. Specifically: identification of epitopes in humoral response (lgG) to Papilomavirus and acute renal vascular rejection (Component A); identification of auto immune CD4+ T cell epitopes involved in the rheumatic fever disease (Component B); inflammation and endothelial cells in the atherosclerotic disease (Component C); B1 Iymphocytes and tumor growth (Component O); thymus and regulatory cells development (Component E); study of the structural behavior of selected peptides and the dynamic of interaction with their respective receptors by nuclear magnetic resonance. Below are the experimental details of each component of the project. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SILVA, ROSEMEIRE A.; GIORDANO, RICARDO J.; GUTIERREZ, PAULO S.; ROCHA, VIVIANE Z.; RUDNICKI, MARTINA; KEE, PATRICK; ABDALLA, DULCINEIA S. P.; PUECH-LEAO, PEDRO; CARAMELLI, BRUNO; ARAP, WADIH; PASQUALINI, RENATA; MENEGHETTI, JOSE C.; MARQUES, FABIO L. N.; KHOOBCHANDANI, MENKA; KATTI, KATTESH V.; LUGAO, ADEMAR B.; KALIL, JORGE. CTHRSSVVC Peptide as a Possible Early Molecular Imaging Target for Atherosclerosis. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 17, n. 9 SEP 2016. Web of Science Citations: 0.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.