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Development of electrochemical biosensor and lateral flow device, low-cost for detection of neoplastic biomarkers

Grant number: 19/10452-2
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): October 01, 2019
Effective date (End): February 28, 2023
Field of knowledge:Health Sciences - Collective Health
Principal Investigator:Maria Del Pilar Taboada Sotomayor
Grantee:Rafael da Fonseca Alves
Home Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Breast cancer (CM) is the second most common type of tumor in the population and the most common type of tumor in women, in the world and in Brazil. The origin of the disease is complex and involves several risk factors, including environmental, biological and hereditary. It is statistically defined as being responsible for 28% of new cases of cancer each year, which puts it on an epidemic scale representing a problem for public health. Currently, CM is diagnosed by imaging and biopsy. The search for more accurate diagnostic methods that detect the disease early has become the starting point for different scientific approaches. The Phage Display technique allows the selection of specific peptides to the most diverse molecular targets. In this context, the construction of biological sensors and Lateral Flow type immunoassays based on ligand peptides selected by Phage Display is shown as a promising diagnostic alternative. This platform stands out for its high sensitivity and selectivity coupled with the possibility of miniaturization, portability, low cost and a minimum amount of energy required for operation. This work aims at the construction of a biosensor from the study of the electrochemical and morphological properties of materials derived from the 3- (3-aminophenyl) propionic acid monomer (3-3-AFP) under the surface of low-cost electrodes and the construction of a lateral flow system using CM-specific binding peptides as the biological recognition interface.