Development of electrochemical biosensor and lateral flow device, low-cost for detection of neoplastic biomarkers

• Grant number: 19/10452-2
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: October 01, 2019
• End date: June 30, 2023
• Field of knowledge: Health Sciences - Collective Health - Public Health
• Principal Investigator: Maria Del Pilar Taboada Sotomayor
• Grantee: Rafael da Fonseca Alves
• Host Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated scholarship(s): 21/12307-0 - Application of recombinant peptides selected by phage display as biorecognition platforms in lateral flow assays for the diagnosis of breast cancer, BE.EP.DR

Abstract

Breast Cancer (CM) is the second most common type of tumor in the population and the most common type of tumor in women, in the world and in Brazil. The origin of the disease is complex and involves several risk factors, including environmental, biological and hereditary. It is statistically defined as being responsible for 28% of new cases of Cancer each year, which puts it on an epidemic scale representing a problem for public health. Currently, CM is diagnosed by imaging and biopsy. The search for more accurate diagnostic methods that detect the disease early has become the starting point for different scientific approaches. The phage display technique allows the selection of specific peptides to the most diverse molecular targets. In this context, the construction of biological sensors and lateral flow type immunoassays based on ligand peptides selected by phage display is shown as a promising diagnostic alternative. This platform stands out for its high sensitivity and selectivity coupled with the possibility of miniaturization, portability, low cost and a minimum amount of energy required for operation. This work aims at the construction of a biosensor from the study of the electrochemical and morphological properties of materials derived from the 3- (3-aminophenyl) propionic acid monomer (3-3-AFP) under the surface of low-cost electrodes and the construction of a lateral flow system using CM-specific binding peptides as the biological recognition interface. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALVES, RAFAEL DA FONSECA; MARTINS, ISABELLA CASTRO; FRANCO, DIEGO LEONI; SILVA, ADRIANE DAS GRACAS; SANTOS, PAULA DE SOUZA; GOULART, LUIZ RICARDO; DE PAIVA MAIA, YARA CRISTINA; FARIA SILVA, ALINNE TATIANE; ARAUJO, THAISE GONCALVES; TABOADA SOTOMAYOR, MARIA DEL PILAR. A novel peptide-based electrochemical biosensor for breast cancer characterization over a poly 3-(3-aminophenyl) propionic acid matrix. BIOSENSORS & BIOELECTRONICS, v. 205, p. 10-pg., 2022-06-01. (19/10452-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ALVES, Rafael da Fonseca. Bioanalytical approaches based on recombinant peptide selected by Phage Display aiming the detection and characterization of breast cancer. 2023. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Química. Araraquara Araraquara.