Hypertonic ressuscitation

• Grant number: 91/03536-8
• Support Opportunities: Research Projects - Thematic Grants
• Start date: June 01, 1992
• End date: July 31, 1996
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Maurício da Rocha e Silva
• Grantee: Maurício da Rocha e Silva
• Host Institution: Instituto do Coração Professor Euryclides de Jesus Zerbini (INCOR). Hospital das Clínicas da Faculdade de Medicina da USP (HCFMUSP). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

In 1976, Prof. Irineu Velasco defended the doctoral thesis entitled 'Hemorrhagic shock: effects of the endovenous administration of hyperosmolar' and its subsequent publication in 1980 are the initial landmark in the development of this new field of basic and applied research, known as: hypertonic resuscitation, or low-volume resuscitation. To-date, there are 167 international publications relating to this theme, of which 69 coming from Brazilian laboratories or Brazilian researchers, active in Brazil and abroad. The theme has been the object of 4 specifically devoted international symposiums (SALT I, San Francisco, USA, 1986; SALT II, Monterrey, USA, 1987; SALT III, Ilha Bela, Brazil, 1988; Garmisch-Patenkirchen, Germany, 1990). Chromosomic segments frequently lost in tumors can be considered as being candidate regions for the location of cancer suppressor genes. In this work, with the initial objective of determining the frequency of the loss of heterozygosis in solid tumors, we will analyze DNA samples of the tumor and the normal tissue of patients with carcinomas of the colon, lung and breast for RFLP analysis using 25 probes of polymorphic DNA distributed in 7 chromosomes. In order to attempt to evaluate the sequence in which the loss of heterozygosis occurs in cancer of the breast, we will use samples of tumors fixed and preserved in blocks of paraffin of patients with a minimum of five years "follow up”. The DNA samples extracted from normal tissue and from the tissue resulting from the dissection of specific stages for each tumor will be enlarged by the PCR method for a panel of highly polymorphic loci within regions which present frequent losses of heterozygosis (determined by the RFLP analysis). (AU)