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Structure of arbuscular mycorhizal fungi associated with vegetal species cultivated in agroforest systems in Central Amazonia


In the present study it is intended to investigate in field conditions the structure of communities of MA fungi under the effect of pupunha (Bactris gasipaes) and cupuassu (Theobroma grandiflorum), cultivated in agroforest system and in monoculture, and of leguminosae cover of tropical kudzu (Pueraria phaseoloides) present in these cultivation systems, as well as the effect of the different patterns of structure observed in the growth of these species, in greenhouse conditions. The field trial is located in Embrapa Western Amazonia, in the municipality of Manaus (MA). We will carry out two collections of soils and roots during a year (dry and wet season). The soil samples will be evaluated for the diversity and density of spores of MA fungi through the classic method of taxonomy. Subsequently, we will undertake, using molecular methods, the confirmation of the species determined in the previous stage. In the root samples we will evaluate the mycorrhizal colonization and molecularly determine the presence of the different species of mycorrhizal fungi observed within the soil. In a greenhouse trial, we will produce the different patterns of communities observed in the field and evaluate their effects on the growth of cupuassu, pupunha and kudzu tropical. In this trial we will determine the levels of nutrients and the dry matter in the exposed and root parts. In the roots we will evaluate the total colonization and the presence of the different species of MA fungi. (AU)

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