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Electron paramagnetic resonance in structural studies of proteins, peptides, biomembranes, polymers, model molecules and their complexes with transition metals

Grant number: 03/09859-2
Support type:PRONEX Research - Thematic Grants
Duration: October 01, 2004 - September 30, 2008
Field of knowledge:Physical Sciences and Mathematics - Physics - Condensed Matter Physics
Cooperation agreement: CNPq - Pronex
Principal researcher:Otaciro Rangel Nascimento
Grantee:Otaciro Rangel Nascimento
Home Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
Pesquisadores principais:
Jose Pedro Donoso Gonzalez

Abstract

Continuous-Wave (CW) Electron Paramagnetic Resonance (EPR) has been widely used in structural studies of a variety of problems, inc1uding many interesting biologically relevant systems. It has contributed very significant1y to the characterization of metal binding sites in proteins, to the study of the dynamic structure of biomembranes and to the kinetics of several processes such as electron transfer. More recent1y, EPR has been experiencing a resurgence especially due to the introduction of new CW methodologies, such as site-directed spin labeling, as well as to its extension to very high frequencies and to the appearance of modem time resolved techniques (pulsed techniques). The latter has taken advantage of the incredible instrumental development achieved during the last few years, thus taking EPR to a new level, where pulsed spectrometers are commercially available. However, unlike NMR, which has almost abolished the continuous wave methods, EPR still makes use of these experiments in a great number of situations. Some very important results have been obtained in systems like metalloproteins and polymers. This project is mainly focused on the applications of EPR to structural studies of proteins, biomembranes, small peptides, model molecules and their complexes with transition metal ions. The establishment of an Electron Paramagnetic Resonance Center congregates several research projects traditionally under investigation in the Molecular Biophysics Group (IFSCIUSP). The use of EPR in its various forms (multifrequencies, CW-EPR, pulsed EPR) is the common point amongst the different projects. The general interests are of physical, chemical and biological relevance. More specifically, the research projects can be divided in three main areas: 1. Biological membranes - involving the projects: Dihydroorotato dehydrogenase from Xylella fastidiosa and its interactions with membranes, Dynamic structure of biologically relevant membranes and peptide-lipid interactions, Permeation process across the capsule of the microalga by EPR technique, and Development of a S-band EPR spectrometer. 2. Metalloproteins and their model systems: involving the projects , Comparative studies of hemeproteins and iron-containing proteins under the influence of conformational modulators, Strucutural studies of non-heme metaloproteins, Transitions ion complexes with small biologically relevant ligands (Cu, Mn, Vo, etc, aminoacids, dipeptides, etc...), and Redox processes and the reaction mechanisms of model porfirins...(AU)

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Scientific publications (15)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
NASCIMENTO, OTACIRO R.; NAPOLITANO, LIA M. B.; TORRE, MARIA H.; PENA, OCTAVIO; CALVO, RAFAEL. EPR and Magnetic Studies of a Carboxylate-Bridged Dinuclear Copper(II) Compound: [Cu-2(flu)(4)(dmf)(2)]. Journal of the Brazilian Chemical Society, v. 22, n. 4, p. 669-676, . (03/09859-2)
LOPES‚ LVS; MACHADO‚ GO; PAWLICKA‚ A.; DONOSO‚ J.P.. Nuclear magnetic resonance and conductivity study of hydroxyethylcellulose based polymer gel electrolytes. Electrochimica Acta, v. 50, n. 19, p. 3978-3984, . (03/09859-2)
FACCHIN‚ G.; KREMER‚ E.; BARAN‚ E.J.; CASTELLANO‚ E.E.; PIRO‚ O.E.; ELLENA‚ J.; COSTA-FILHO‚ A.J.; TORRE‚ M.H.. Structural characterization of a series of new Cu-dipeptide complexes in solid state and in solution. Polyhedron, v. 25, n. 13, p. 2597-2604, . (03/09859-2)
TAMBELLI‚ CE; DONOSO‚ JP; MAGON‚ CJ; PEREIRA‚ EC; ROSARIO‚ AV. NMR‚ conductivity and DSC study of Li+ transport in ethylene glycol/citric acid polymer gel. Electrochimica Acta, v. 53, n. 4, p. 1535-1540, . (03/09859-2)
MARMO MOREIRA‚ L.; LIMA POLI‚ A.; COSTA-FILHO‚ A.J.; IMASATO‚ H.. Pentacoordinate and hexacoordinate ferric hemes in acid medium: EPR‚ UV-Vis and CD studies of the giant extracellular hemoglobin of Glossoscolex paulistus. Biophysical Chemistry, v. 124, n. 1, p. 62-72, . (03/09859-2)
CAMPOS-BERMUDEZ, VALERIA A.; MORAN-BARRIO, JORGELINA; COSTA-FILHO, ANTONIO J.; VILA, ALEJANDRO J.. Metal-dependent inhibition of glyoxalase II: A possible mechanism to regulate the enzyme activity. Journal of Inorganic Biochemistry, v. 104, n. 7, p. 726-731, . (03/09859-2)
MAIA, PEDRO I. DA S.; PAVAN, FERNANDO R.; LEITE, CLARICE Q. F.; LEMOS, SEBASTIAO S.; DE SOUSA, GERIMARIO F.; BATISTA, ALZIR A.; NASCIMENTO, OTACIRO R.; ELLENA, JAVIER; CASTELLANO, EDUARDO E.; NIQUET, ELKE; et al. Vanadium complexes with thiosemicarbazones: Synthesis, characterization, crystal structures and anti-Mycobacterium tuberculosis activity. Polyhedron, v. 28, n. 2, p. 398-406, . (03/09859-2)
CITADINI‚ A.P.S.; PINTO‚ A.; ARAÚJO‚ A.P.U.; NASCIMENTO‚ O.R.; COSTA-FILHO‚ A.J.. EPR studies of chlorocatechol 1‚ 2-dioxygenase: evidences of iron reduction during catalysis and of the binding of amphipatic molecules. BIOPHYSICAL JOURNAL, v. 88, n. 5, p. 3502-3508, . (03/09859-2)
DONOSO‚ JP; LOPES‚ LVS; PAWLICKA‚ A.; FUENTES‚ S.; RETUERT‚ PJ; GONZÁLEZ‚ G.. Nuclear magnetic resonance study of PEO-chitosan based polymer electrolytes. Electrochimica Acta, v. 53, n. 4, p. 1455-1460, . (03/09859-2)
VIEIRA‚ E.D.; CASADO‚ N.M.C.; FACCHIN‚ G.; TORRE‚ M.H.; COSTA-FILHO‚ A.J.; CALVO‚ R.. Weak exchange interaction supported by a biologically relevant long chemical bridge in a Cu-peptide model compound. Inorganic Chemistry, v. 45, n. 7, p. 2942-2947, . (03/09859-2)
ELLENA‚ J.; KREMER‚ E.; FACCHIN‚ G.; BARAN‚ E.J.; NASCIMENTO‚ O.R.; COSTA-FILHO‚ A.J.; TORRE‚ M.H.. X-ray structure and EPR behavior of a new dimeric copper (II) complex with 4-amino- N-(5-methoxy-2-pyrimidinyl) benzenesulfonamide. Polyhedron, v. 26, n. 13, p. 3277-3285, . (03/09859-2)
MATTOS‚ RI; PAWLICKA‚ A.; LIMA‚ JF; TAMBELLI‚ CE; MAGON‚ CJ; DONOSO‚ JP. Magnetic resonance and conductivity study of gelatin-based proton conductor polymer electrolytes. Electrochimica Acta, v. 55, n. 4, p. 1396-1400, . (03/09859-2)
COUTO‚ S.G.; NONATO‚ M.C.; COSTA-FILHO‚ A.J.. Defects in vesicle core induced by Escherichia coli dihydroorotate dehydrogenase. BIOPHYSICAL JOURNAL, v. 94, n. 5, p. 1746-1753, . (03/09859-2)
NAPOLITANO‚ L.M.B.; NASCIMENTO‚ O.R.; CABALEIRO‚ S.; CASTRO‚ J.; CALVO‚ R.. Isotropic and anisotropic spin-spin interactions and a quantum phase transition in a dinuclear Cu (II) compound. Physical Review B, v. 77, n. 21, p. 214423, . (03/09859-2)
VIEIRA, ERNANNI D.; FACCHIN, GIANELLA; TORRE, MARIA H.; COSTA-FILHO, ANTONIO J.. Exchange interactions in the copper(II)-N-benzoylglycine (hippuric acid) complex. Journal of the Brazilian Chemical Society, v. 19, n. 8, p. 1614-1621, . (03/09859-2)

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