Repair of damaged DNA and biological consequences

• Grant number: 98/11119-7
• Support Opportunities: Research Projects - Thematic Grants
• Start date: January 01, 1999
• End date: February 29, 2004
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Carlos Frederico Martins Menck
• Grantee: Carlos Frederico Martins Menck
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Pesquisadores principais: Márcio de Castro Silva Filho ; Marie-Anne Van Sluys
• Associated scholarship(s): 01/00963-6 - Complementation of the DNA repair deficiency in human cells by transducing the gene XPC cDNA with recombinant adenovirus, BP.MS; 01/01728-0 - Complementation of the DNA repair deficiency in human cells by transducing the gene XPA cDNA with recombinant adenovirus, BP.PD; 99/03419-3 - Complementation of the DNA repair deficiency in human cells by transferring the gene XPC with recombinant adenovirus, BP.IC

Abstract

The proposal aims to study different aspects of the Molecular Biology of the repair of injured DNA in various systems. In animal cells we are developing genetic vectors to intervene in the repair system and thus obtain information on how these act on some levels, such as, for example, apoptosis, mutagenesis and recombination. In plants we are trying to identify functions of two genes (araXPB and thi1) cloned by us in the repair of DNA, either through in vitro trials or in the construction of transgenetic plants modified by these genes. Finally, we are continuing the previous work in which we studied the repair mechanisms of bacterial DNA responsible for the protection of the genome against lesions induced by singlet oxygen. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

AGNEZ-LIMA‚ LF; NAPOLITANO‚ RL; FUCHS‚ RPP; DI MASCIO‚ P.; MUOTRI‚ AR; MENCK‚ CFM. DNA repair and sequence context affect 1O2-induced mutagenesis in bacteria. Nucleic Acids Research, v. 29, n. 13, p. 2899-2903, 2001. (98/11119-7)

MUOTRI‚ A.R.; MARCHETTO‚ M.C.N.; SUZUKI‚ M.F.; OKAZAKI‚ K.; LOTFI‚ C.F.P.; BRUMATTI‚ G.; AMARANTE-MENDES‚ G.P.; MENCK‚ C.F.M.. Low amounts of the DNA repair XPA protein are sufficient to recover UV-resistance. Carcinogenesis, v. 23, n. 6, p. 1039-1046, 2002. (98/11119-7)

MUOTRI‚ A.R.; MARCHETTO‚ M.C.N.; ZERBINI‚ L.F.C.; LIBERMANN‚ T.A.; VENTURA‚ A.M.; SARASIN‚ A.; MENCK‚ C.F.M.. Complementation of the DNA repair deficiency in human xeroderma pigmentosum group A and C cells by recombinant adenovirus-mediated gene transfer. Human Gene Therapy, v. 13, n. 15, p. 1833-1844, 2002. (98/11119-7)

JORGE‚ S.A.C.; MENCK‚ C.F.M.; SIES‚ H.; OSBORNE‚ M.R.; PHILLIPS‚ D.H.; SARASIN‚ A.; STARY‚ A.. Mutagenic fingerprint of ozone in human cells. DNA Repair, v. 1, n. 5, p. 369-378, 2002. (98/11119-7)

CAVALCANTE‚ A.K.D.; MARTINEZ‚ G.R.; DI MASCIO‚ P.; MENCK‚ C.F.M.; AGNEZ-LIMA‚ L.F.. Cytotoxicity and mutagenesis induced by singlet oxygen in wild type and DNA repair deficient Escherichia coli strains. DNA Repair, v. 1, n. 12, p. 1051-1056, 2002. (00/03878-7, 98/11119-7)

CHIGANÇAS‚ V.; MIYAJI‚ E.N.; MUOTRI‚ A.R.; JACYSYN‚ J.F.; AMARANTE-MENDES‚ G.P.; YASUI‚ A.; MENCK‚ C.F.M.. Photorepair prevents ultraviolet-induced apoptosis in human cells expressing the marsupial photolyase gene. Cancer Research, v. 60, n. 9, p. 2458, 2000. (96/04518-7, 98/11119-7)

CHABREGAS, SABRINA M.; LUCHE, DOUGLAS D.; FARIAS, LEONARDO P.; RIBEIRO, ALBERTO F.; VAN SLUYS, MARIE-ANNE; MENCK, CARLOS F. M.; SILVA-FILHO, MARCIO C.. Dual targeting properties of the N-terminal signal sequence of Arabidopsis thaliana THI1 protein to mitochondria and chloroplasts. Plant Molecular Biology, v. 46, n. 6, p. 639-650, 2001. (98/11119-7)

CHABREGAS, SABRINA M.; LUCHE, DOUGLAS D.; VAN SLUYS, MARIE-ANNE; MENCK, CARLOS F. M.; SILVA-FILHO, MARCIO C.. Differential usage of two in-frame translational start codons regulates subcellular localization of Arabidopsis thaliana THI1. Journal of Cell Science, v. 116, n. 2, p. 285-291, Jan. 2003. (98/11119-7)