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Comparative physiology of peripheral clocks: clock genes (CLOCK, PER1, PER2, CRY1 and BMAL 1) and their modulation by light and hormones in fish, amphibians and mammals

Abstract

Melanopsin can be expressed, as well as other opsins, in skin pigment cells, to mediate photoresponses. Having in mind that cultured cells may constitute peripheral clocks and respond to visible light, we will analyse: 1. the mechanisms to adjust biological clocks in single cells of teleosts, amphibians and mammals; 2. their regulation by varying photoperiod regimes and hormones; We will investigate: 1. whether ZEM-2S embryonic cell line of Danio rerio, melanophores of Xenopus laevis, and B-16 F10 murine melanoma cells are able to cycle genes such as Per1, independently of light:dark cycle; 2. whether opsin expression is rhythmic, dependent on the integrity of the cellular clock and independent of the light:dark cycle; 3. whether opsin and clock gene expressions may be modified by hormones. These hypotheses will be tested by the quantification of luciferin bioluminescence originated from the activation of luciferase located in Per1 promoter, of mRNA (and of proteins whenever possible) of opsinsand clock genes, under light:dark cycles, or constant darkness, in the presence of increasing hormone (melatonin, endothelin and alfa-MSH) concentrations, for increasing periods of time. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ISOLDI, MAURO CESAR; PROVENCIO, IGNACIO; DE LAURO CASTRUCCI, ANA MARIA. Light modulates the melanophore response to alpha-MSH in Xenopus laevis: An analysis of the signal transduction crosstalk mechanisms involved. General and Comparative Endocrinology, v. 165, n. 1, p. 104-110, JAN 1 2010. Web of Science Citations: 8.

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