| Grant number: | 12/50214-4 |
| Support Opportunities: | Research Projects - Thematic Grants |
| Start date: | December 01, 2012 |
| End date: | November 30, 2017 |
| Field of knowledge: | Biological Sciences - Physiology - Compared Physiology |
| Principal Investigator: | Ana Maria de Lauro Castrucci |
| Grantee: | Ana Maria de Lauro Castrucci |
| Host Institution: | Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil |
| City of the host institution: | São Paulo |
| Associated scholarship(s): | 14/16412-9 - Mechanisms of clock genes thermo-modulation in peripheral tissue of mammals: Role of TRP channels,
BP.PD 13/02131-5 - EFFECTS OF LIGHT AND TEMPERATURE ON CLOCK GENES EXPRESSION IN AMPHIBIANS AND MAMMALS: THE PIGMENT CELL AS A STUDY MODEL, BP.MS 13/24337-4 - CLOCK GENES MODULATION BY UVA/BLUE LIGHT STIMULATION IN NORMAL MELAN-A AND TRANSFORMED B-16 MELANOCYTES, BP.DD |
Abstract
Our research group has been investigating the physiology of photopigments and clock genes in cell models of various vertebrate classes since 2001. During this period the group has published several findings on the mechanism of action of light and hormones on the expression of the photopigment melanopsin and of clock genes in each of our models. The present project arises as the continuation of the perspective adopted in our laboratory, and aims to widen the study of the sensorial pathways investigated in our previous projects in order to enlighten the interaction of light and temperature detection systems with the circadian system. Our objectives are: (1) to investigate light and temperature as synchronizing agents of the clock (expression of clock genes by Lumicycle and/or quantitative PCR) in teleostean, anphibian, avian, and mammalian cell lines; (2) to identify the TRP channels affected by light and temperature (by nanostring and/or quantitative PCR) in teleostean, anphibian, avian, and mammalian cell lines; (3) to identify the mechanisms assessing: a) the effect of TRP channel blockade with antagonists and/or with silencing by RNAi on clock genes; b) the effect of melanopsins silencing by RNAi on clock genes; c) the effect of the scaffold protein INAD blockade on clock genes; (4) to assess in in vivo models of homeoterms (Mus musculus) the role of TRP V1 channel using antagonist i.p. injection and quantifying mRNA of clock genes in peripheral tissues of resting and exercising animals; (5) to assess in in vivo models of ectoterms (Danio rerio) the role of TRP channels quantifying mRNA of clock genes in peripheral tissues of wild type and TRP knockout animals submitted to temperature variation. (AU)
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