Biological clock setting by light and temperature: phylogenetic aspects

• Grant number: 12/50214-4
• Support Opportunities: Research Projects - Thematic Grants
• Duration: December 01, 2012 - November 30, 2017
• Field of knowledge: Biological Sciences - Physiology - Compared Physiology
• Principal Investigator: Ana Maria de Lauro Castrucci
• Grantee: Ana Maria de Lauro Castrucci
• Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 14/16412-9 - Mechanisms of clock genes thermo-modulation in peripheral tissue of mammals: role of TRP channels, BP.PD; 13/02131-5 - Effects of light and temperature on clock genes expression in amphibians and mammals: the pigment cell as a study model, BP.MS; 13/24337-4 - Clock genes modulation by UVA/blue light stimulation in normal melan-A and transformed B-16 melanocytes, BP.DD

Abstract

Our research group has been investigating the physiology of photopigments and clock genes in cell models of various vertebrate classes since 2001. During this period the group has published several findings on the mechanism of action of light and hormones on the expression of the photopigment melanopsin and of clock genes in each of our models. The present project arises as the continuation of the perspective adopted in our laboratory, and aims to widen the study of the sensorial pathways investigated in our previous projects in order to enlighten the interaction of light and temperature detection systems with the circadian system. Our objectives are: (1) to investigate light and temperature as synchronizing agents of the clock (expression of clock genes by Lumicycle and/or quantitative PCR) in teleostean, anphibian, avian, and mammalian cell lines; (2) to identify the TRP channels affected by light and temperature (by nanostring and/or quantitative PCR) in teleostean, anphibian, avian, and mammalian cell lines; (3) to identify the mechanisms assessing: a) the effect of TRP channel blockade with antagonists and/or with silencing by RNAi on clock genes; b) the effect of melanopsins silencing by RNAi on clock genes; c) the effect of the scaffold protein INAD blockade on clock genes; (4) to assess in in vivo models of homeoterms (Mus musculus) the role of TRP V1 channel using antagonist i.p. injection and quantifying mRNA of clock genes in peripheral tissues of resting and exercising animals; (5) to assess in in vivo models of ectoterms (Danio rerio) the role of TRP channels quantifying mRNA of clock genes in peripheral tissues of wild type and TRP knockout animals submitted to temperature variation. (AU)

Scientific publications

(22)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE ASSIS, L. V. M.; MORAES, M. N.; DA SILVEIRA CRUZ-MACHADO, S.; CASTRUCCI, A. M. L.. The effect of white light on normal and malignant murine melanocytes: A link between opsins, clock genes, and melanogenesis. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, v. 1863, n. 6, A, p. 1119-1133, JUN 2016. (13/24337-4, 12/50214-4, 15/04557-5, 14/16412-9)

MONTEIRO DE ASSIS, LEONARDO VINICIUS; MORAES, MARIA NATHALIA; MAGALHAES-MARQUES, KEILA KAROLINE; KINKER, GABRIELA SARTI; CRUZ-MACHADO, SANSERAY DA SILVEIRA; DE LAURO CASTRUCCI, ANA MARIA. Non-Metastatic Cutaneous Melanoma Induces Chronodisruption in Central and Peripheral Circadian Clocks. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 19, n. 4, APR 2018. (12/50214-4)

SOUSA, JENNIFER CAROLINE; MAGALHAES-MARQUES, KEILA KAROLINE; CRUZ-MACHADO, SANSERAY DA SILVEIRA; MORAES, MARIA NATHALIA; DE LAURO CASTRUCCI, ANA MARIA. Dexamethasone Modulates Nonvisual Opsins, Glucocorticoid Receptor, and Clock Genes in Danio rerio ZEM-2S Cells. BIOMED RESEARCH INTERNATIONAL, 2017. (12/50214-4)

MONTEIRO DE ASSIS, LEONARDO VINICIUS; KINKER, GABRIELA SARTI; MORAES, MARIA NATHALIA; MARKUS, REGINA P.; FERNANDES, PEDRO AUGUSTO; DE LAURO CASTRUCCI, ANA MARIA. Expression of the Circadian Clock Gene BMAL1 Positively Correlates With Antitumor Immunity and Patient Survival in Metastatic Melanoma. FRONTIERS IN ONCOLOGY, v. 8, JUN 12 2018. (13/24337-4, 14/27287-0, 12/50214-4, 14/16412-9)

MONTEIRO DE ASSIS, LEONARDO VINCIUS; MORAES, MARIA NATHALIA; DE LAURO CASTRUCCI, ANA MARIA. Heat shock antagonizes UVA-induced responses in murine melanocytes and melanoma cells: an unexpected interaction. PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, v. 16, n. 5, p. 633-648, MAY 2017. (12/50214-4)

MORAES, MARIA NATHALIA; RAMOS, BRUNO C.; POLETINI, MARISTELA O.; CASTRUCCI, ANA MARIA L.. Melanopsins: Localization and Phototransduction in Xenopus laevis Melanophores. Photochemistry and Photobiology, v. 91, n. 5, p. 1133-1141, SEP-OCT 2015. (12/50214-4)

MORAES, MARIA NATHALIA; MONTEIRO DE ASSIS, LEONARDO VINICIUS; HENRIQUES, FELIPE DOS SANTOS; BATISTA, JR., MIGUEL LUIZ; GULER, ALI D.; DE LAURO CASTRUCCI, ANA MARIA. Cold-sensing TRPM8 channel participates in circadian control of the brown adipose tissue. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, v. 1864, n. 12, p. 2415-2427, DEC 2017. (12/50214-4)

MORAES, MARIA NATHLIA; MONTEIRO DE ASSIS, LEONARDO VINICIUS; MAGALHAES-MARQUES, KEILA KAROLINE; POLETINI, MARISTELA OLIVEIRA; RIBEIRO GRACIANI DE LIMA, LEONARDO HENRIQUE; DE LAURO CASTRUCCI, ANA MARIA. Melanopsin, a Canonical Light Receptor, Mediates Thermal Activation of Clock Genes. SCIENTIFIC REPORTS, v. 7, OCT 25 2017. (12/50214-4)

SOUSA, JENNIFER CAROLINE; MAGALHAES-MARQUES, KEILA KAROLINE; CRUZ-MACHADO, SANSERAY DA SILVEIRA; MORAES, MARIA NATHALIA; DE LAURO CASTRUCCI, ANA MARIA. Dexamethasone Modulates Nonvisual Opsins, Glucocorticoid Receptor, and Clock Genes in Danio rerio ZEM-2S Cells. BIOMED RESEARCH INTERNATIONAL, v. 2017, p. 14-pg., 2017-01-01. (12/50214-4)

JERONIMO, RODRIGO; MORAES, MARIA NATHALIA; MONTEIRO DE ASSIS, LEONARDO VINICIUS; RAMOS, BRUNO CESAR; ROCHA, THAINA; DE LAURO CASTRUCCI, ANA MARIA. Thermal stress in Danio rerio: a link between temperature, light, thermo-TRP channels, and clock genes. Journal of Thermal Biology, v. 68, p. 11-pg., 2017-08-01. (12/50214-4, 14/16412-9, 13/24337-4)

JERONIMO, RODRIGO; MORAES, MARIA NATHALIA; MONTEIRO DE ASSIS, LEONARDO VINICIUS; RAMOS, BRUNO CESAR; ROCHA, THAINA; DE LAURO CASTRUCCI, ANA MARIA. Thermal stress in Danio rerio: a link between temperature, light, thermo-TRP channels, and clock genes. Journal of Thermal Biology, v. 68, n. A, p. 128-138, AUG 2017. (12/50214-4)

POLETINI, MARISTELA O.; RAMOS, BRUNO C.; MORAES, MARIA NATHALIA; CASTRUCCI, ANA MARIA L.. Nonvisual Opsins and the Regulation of Peripheral Clocks by Light and Hormones. Photochemistry and Photobiology, v. 91, n. 5, p. 1046-1055, SEP-OCT 2015. (12/50214-4)

MONTEIRO DE ASSIS, LEONARDO VINICIUS; MORAES, MARIA NATHALIA; MAGALHAES-MARQUES, KEILA KAROLINE; DE LAURO CASTRUCCI, ANA MARIA. Melanopsin and rhodopsin mediate UVA-induced immediate pigment darkening: Unravelling the photosensitive system of the skin. EUROPEAN JOURNAL OF CELL BIOLOGY, v. 97, n. 3, p. 150-162, APR 2018. (14/16412-9, 13/24337-4, 12/50214-4)

MONTEIRO DE ASSIS, LEONARDO VINICIUS; MORAES, MARIA NATHALIA; DE LAURO CASTRUCCI, ANA MARIA. The molecular clock in the skin, its functionality, and how it is disrupted in cutaneous melanoma: a new pharmacological target?. CELLULAR AND MOLECULAR LIFE SCIENCES, v. 76, n. 19, p. 3801-3826, OCT 2019. (12/50214-4, 18/14728-0)

MORAES, MARIA NATHALIA; MEZZALIRA, NATHANA; MONTEIRO DE ASSIS, LEONARDO VINICIUS; MENAKER, MICHAEL; GULER, ALI; CASTRUCCI, ANA MARIA L.. TRPV1 participates in the activation of clock molecular machinery in the brown adipose tissue in response to light-dark cycle. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, v. 1864, n. 2, p. 324-335, FEB 2017. (12/50214-4)

POLETINI, MARISTELA OLIVEIRA; DE ASSIS, LEONARDO VINICIUS MONTEIRO; MORAES, MARIA NATHALIA; DE LAURO CASTRUCCI, ANA MARIA. Estradiol differently affects melanin synthesis of malignant and normal melanocytes: a relationship with clock and clock-controlled genes. Molecular and Cellular Biochemistry, v. 421, n. 1-2, p. 29-39, OCT 2016. (12/50214-4)

MONTEIRO DE ASSIS, LEONARDO VINICIUS; MENDES, DAVI; SILVA, MATHEUS MOLINA; KINKER, GABRIELA SARTI; PEREIRA-LIMA, ISABELLA; MORAES, MARIA NATHALIA; MARTINS MENCK, CARLOS FREDERICO; DE LAURO CASTRUCCI, ANA MARIA. Melanopsin mediates UVA-dependent modulation of proliferation, pigmentation, apoptosis, and molecular clock in normal and malignant melanocytes. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, v. 1867, n. 10, OCT 2020. (14/27287-0, 19/19435-3, 13/24337-4, 17/24615-5, 12/50214-4, 18/16511-8, 17/26651-9, 17/18781-0, 18/14728-0, 17/24217-0)

LACERDA, JOSE THALLES; GOMES, PATRICIA R. L.; ZANETTI, GIOVANNA; MEZZALIRA, NATHANA; LIMA, OTONIEL G.; DE ASSIS, LEONARDO V. M.; GULER, ALI; CASTRUCCI, ANA MARIA; MORAES, MARIA NATHALIA. Lack of TRPV1 Channel Modulates Mouse Gene Expression and Liver Proteome with Glucose Metabolism Changes. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 23, n. 13, p. 24-pg., 2022-07-01. (17/24615-5, 21/01659-2, 14/16412-9, 20/04524-8, 18/23915-8, 18/16511-8, 13/02131-5, 12/50214-4, 18/14728-0, 17/26651-9, 21/09923-0)

MONTEIRO DE ASSIS, LEONARDO VINICIUS; LACERDA, JOSE THALLES; MORAES, MARIA NATHALIA; DOMINGUEZ-AMOROCHO, OMAR ALBERTO; KINKER, GABRIELA SARTI; MENDES, DAVI; SILVA, MATHEUS MOLINA; MARTINS MENCK, CARLOS FREDERICO; SARAIVA CAMARA, NIELS OLSEN; DE LAURO CASTRUCCI, ANA MARIA. Melanopsin (Opn4) is an oncogene in cutaneous melanoma. COMMUNICATIONS BIOLOGY, v. 5, n. 1, p. 15-pg., 2022-05-13. (19/19435-3, 19/25129-2, 17/24217-0, 17/26651-9, 18/16511-8, 12/50214-4, 18/14728-0, 17/24615-5, 17/18781-0, 20/04524-8, 13/24337-4, 17/16711-4)

SUA-CESPEDES, CRISTHIAN; LACERDA, JOSE THALLES; ZANETTI, GIOVANNA; DAVID, DANIELA DANTAS; MORAES, MARIA NATHALIA; DE ASSIS, LEONARDO V. M.; CASTRUCCI, ANA MARIA L.. Melanopsin (OPN4) is a novel player in skin homeostasis and attenuates UVA-induced effects. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 242, p. 15-pg., 2023-04-03. (16/00696-3, 13/07937-8, 19/19005-9, 21/01659-2, 17/26651-9, 18/23043-0, 18/16511-8, 12/50214-4, 12/12663-1, 18/14728-0, 17/24615-5, 20/04524-8, 14/16412-9)

DE ASSIS, L. V. M.; MORAES, M. N.; DA SILVEIRA CRUZ-MACHADO, S.; CASTRUCCI, A. M. L.. The effect of white light on normal and malignant murine melanocytes: A link between opsins, clock genes, and melanogenesis. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, v. 1863, n. 6, p. 15-pg., 2016-06-01. (12/50214-4, 14/16412-9, 15/04557-5, 13/24337-4)

MONTEIRO DE ASSIS, LEONARDO VINICIUS; MORAES, MARIA NATHALIA; MAGALHAES-MARQUES, KEILA KAROLINE; KINKER, GABRIELA SARTI; CRUZ-MACHADO, SANSERAY DA SILVEIRA; DE LAURO CASTRUCCI, ANA MARIA. Non-Metastatic Cutaneous Melanoma Induces Chronodisruption in Central and Peripheral Circadian Clocks. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 19, n. 4, p. 24-pg., 2018-04-01. (12/50214-4, 15/04557-5, 14/16412-9, 13/24337-4)