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Mechanisms of clock genes thermo-modulation in peripheral tissue of mammals: role of TRP channels

Grant number: 14/16412-9
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): January 01, 2015
Effective date (End): December 31, 2017
Field of knowledge:Biological Sciences - Physiology
Principal Investigator:Ana Maria de Lauro Castrucci
Grantee:Maria Nathália de Carvalho Magalhães Moraes Figueira Borges
Home Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:12/50214-4 - Biological clock setting by light and temperature: phylogenetic aspects, AP.TEM

Abstract

Daily cycles of light and temperature are two major signals of environmental time for the organisms. It is important to emphasize, however, that ambient temperature does not alter body temperature of endothermic animals, as the inner temperature is controlled by the master clock, through light signaling via the retina. As the suprachiasmatic nuclei are entrained by the light-dark cycles, an efferent path from these nuclei activates the pre-optic area responsible for temperature control generating a rhythmic response. In addition, recent work has demonstrated the interaction of opsins with TRP channels in photo-responses as well as responses to temperature variation. Based on these information, we hypothesize that peripheral clocks in the various organs are informed of temperature via mechanisms which culminate in TRP channel activation, and that this signal is used to synchronize the clock. To test this hypothesis, we propose to investigate the effect of temperature on the expression of clock genes in the mouse melanocyte line, Melan-A, which expresses rhodopsin, and investigate the presence and role of opsins and thermo-responsive TRP channels. We will investigate the function of the TRP channels with the use of antagonists, and with permanent silencing by shRNA. In vivo experiments will also be performed, utilizing TRP channel knockout mice and assessing clock genes expression in various peripheral organs in animals subject to various light and temperature regimens. (AU)

Matéria(s) publicada(s) na Agência FAPESP sobre a bolsa:
Investigation of body clock could point to target for treatment of skin cancer 

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MONTEIRO DE ASSIS, LEONARDO VINICIUS; KINKER, GABRIELA SARTI; MORAES, MARIA NATHALIA; MARKUS, REGINA P.; FERNANDES, PEDRO AUGUSTO; DE LAURO CASTRUCCI, ANA MARIA. Expression of the Circadian Clock Gene BMAL1 Positively Correlates With Antitumor Immunity and Patient Survival in Metastatic Melanoma. FRONTIERS IN ONCOLOGY, v. 8, JUN 12 2018. Web of Science Citations: 6.
MONTEIRO DE ASSIS, LEONARDO VINICIUS; MORAES, MARIA NATHALIA; MAGALHAES-MARQUES, KEILA KAROLINE; DE LAURO CASTRUCCI, ANA MARIA. Melanopsin and rhodopsin mediate UVA-induced immediate pigment darkening: Unravelling the photosensitive system of the skin. EUROPEAN JOURNAL OF CELL BIOLOGY, v. 97, n. 3, p. 150-162, APR 2018. Web of Science Citations: 7.
DE ASSIS, L. V. M.; MORAES, M. N.; DA SILVEIRA CRUZ-MACHADO, S.; CASTRUCCI, A. M. L. The effect of white light on normal and malignant murine melanocytes: A link between opsins, clock genes, and melanogenesis. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, v. 1863, n. 6, A, p. 1119-1133, JUN 2016. Web of Science Citations: 19.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.