Mechanisms of clock genes thermo-modulation in peripheral tissue of mammals: role of TRP channels

• Grant number: 14/16412-9
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Effective date (Start): January 01, 2015
• Effective date (End): December 31, 2017
• Field of knowledge: Biological Sciences - Physiology - Compared Physiology
• Principal Investigator: Ana Maria de Lauro Castrucci
• Grantee: Maria Nathália de Carvalho Magalhães Moraes Figueira Borges
• Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 12/50214-4 - Biological clock setting by light and temperature: phylogenetic aspects, AP.TEM

Abstract

Daily cycles of light and temperature are two major signals of environmental time for the organisms. It is important to emphasize, however, that ambient temperature does not alter body temperature of endothermic animals, as the inner temperature is controlled by the master clock, through light signaling via the retina. As the suprachiasmatic nuclei are entrained by the light-dark cycles, an efferent path from these nuclei activates the pre-optic area responsible for temperature control generating a rhythmic response. In addition, recent work has demonstrated the interaction of opsins with TRP channels in photo-responses as well as responses to temperature variation. Based on these information, we hypothesize that peripheral clocks in the various organs are informed of temperature via mechanisms which culminate in TRP channel activation, and that this signal is used to synchronize the clock. To test this hypothesis, we propose to investigate the effect of temperature on the expression of clock genes in the mouse melanocyte line, Melan-A, which expresses rhodopsin, and investigate the presence and role of opsins and thermo-responsive TRP channels. We will investigate the function of the TRP channels with the use of antagonists, and with permanent silencing by shRNA. In vivo experiments will also be performed, utilizing TRP channel knockout mice and assessing clock genes expression in various peripheral organs in animals subject to various light and temperature regimens. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE ASSIS, L. V. M.; MORAES, M. N.; DA SILVEIRA CRUZ-MACHADO, S.; CASTRUCCI, A. M. L.. The effect of white light on normal and malignant murine melanocytes: A link between opsins, clock genes, and melanogenesis. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, v. 1863, n. 6, A, p. 1119-1133, JUN 2016. (13/24337-4, 12/50214-4, 15/04557-5, 14/16412-9)

MONTEIRO DE ASSIS, LEONARDO VINICIUS; KINKER, GABRIELA SARTI; MORAES, MARIA NATHALIA; MARKUS, REGINA P.; FERNANDES, PEDRO AUGUSTO; DE LAURO CASTRUCCI, ANA MARIA. Expression of the Circadian Clock Gene BMAL1 Positively Correlates With Antitumor Immunity and Patient Survival in Metastatic Melanoma. FRONTIERS IN ONCOLOGY, v. 8, JUN 12 2018. (13/24337-4, 14/27287-0, 12/50214-4, 14/16412-9)

JERONIMO, RODRIGO; MORAES, MARIA NATHALIA; MONTEIRO DE ASSIS, LEONARDO VINICIUS; RAMOS, BRUNO CESAR; ROCHA, THAINA; DE LAURO CASTRUCCI, ANA MARIA. Thermal stress in Danio rerio: a link between temperature, light, thermo-TRP channels, and clock genes. Journal of Thermal Biology, v. 68, p. 11-pg., 2017-08-01. (12/50214-4, 14/16412-9, 13/24337-4)

MONTEIRO DE ASSIS, LEONARDO VINICIUS; MORAES, MARIA NATHALIA; MAGALHAES-MARQUES, KEILA KAROLINE; DE LAURO CASTRUCCI, ANA MARIA. Melanopsin and rhodopsin mediate UVA-induced immediate pigment darkening: Unravelling the photosensitive system of the skin. EUROPEAN JOURNAL OF CELL BIOLOGY, v. 97, n. 3, p. 150-162, APR 2018. (14/16412-9, 13/24337-4, 12/50214-4)

LACERDA, JOSE THALLES; GOMES, PATRICIA R. L.; ZANETTI, GIOVANNA; MEZZALIRA, NATHANA; LIMA, OTONIEL G.; DE ASSIS, LEONARDO V. M.; GULER, ALI; CASTRUCCI, ANA MARIA; MORAES, MARIA NATHALIA. Lack of TRPV1 Channel Modulates Mouse Gene Expression and Liver Proteome with Glucose Metabolism Changes. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 23, n. 13, p. 24-pg., 2022-07-01. (17/24615-5, 21/01659-2, 14/16412-9, 20/04524-8, 18/23915-8, 18/16511-8, 13/02131-5, 12/50214-4, 18/14728-0, 17/26651-9, 21/09923-0)

SUA-CESPEDES, CRISTHIAN; LACERDA, JOSE THALLES; ZANETTI, GIOVANNA; DAVID, DANIELA DANTAS; MORAES, MARIA NATHALIA; DE ASSIS, LEONARDO V. M.; CASTRUCCI, ANA MARIA L.. Melanopsin (OPN4) is a novel player in skin homeostasis and attenuates UVA-induced effects. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 242, p. 15-pg., 2023-04-03. (16/00696-3, 13/07937-8, 19/19005-9, 21/01659-2, 17/26651-9, 18/23043-0, 18/16511-8, 12/50214-4, 12/12663-1, 18/14728-0, 17/24615-5, 20/04524-8, 14/16412-9)

DE ASSIS, L. V. M.; MORAES, M. N.; DA SILVEIRA CRUZ-MACHADO, S.; CASTRUCCI, A. M. L.. The effect of white light on normal and malignant murine melanocytes: A link between opsins, clock genes, and melanogenesis. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, v. 1863, n. 6, p. 15-pg., 2016-06-01. (12/50214-4, 14/16412-9, 15/04557-5, 13/24337-4)

MONTEIRO DE ASSIS, LEONARDO VINICIUS; MORAES, MARIA NATHALIA; MAGALHAES-MARQUES, KEILA KAROLINE; KINKER, GABRIELA SARTI; CRUZ-MACHADO, SANSERAY DA SILVEIRA; DE LAURO CASTRUCCI, ANA MARIA. Non-Metastatic Cutaneous Melanoma Induces Chronodisruption in Central and Peripheral Circadian Clocks. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 19, n. 4, p. 24-pg., 2018-04-01. (12/50214-4, 15/04557-5, 14/16412-9, 13/24337-4)