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Cold challenge and its effects on canonical thermosensation processes in BAT and their possible interaction with TRP channels

Grant number: 18/23915-8
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): July 01, 2019
Effective date (End): March 31, 2021
Field of knowledge:Biological Sciences - Physiology - General Physiology
Principal Investigator:Ana Maria de Lauro Castrucci
Grantee:Otoniel Gonçalves de Lima
Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil


In recent years much has been discussed about the importance of transient receptor potential channels (TRP), their relationship with the circadian clock and modulation of physiological processes. Studies showed the association of TRP channels with thermoregulation, metabolism of lipids and the existence of possible links with the circadian clock modulating metabolic processes. In this way the present project proposes to investigate the following hypothesis: the transient receptor potential channels (TRPA1) are required for the cold reception and plasma level increase of natriuretic peptide hormones (NPs) released by the heart which will modulate the expression of NP receptors and clock genes in the brown adipose tissue (BAT), as well as their thermogenic function. We will evaluate the thermal sensation, the local clock and the canonical processes tissue-specific in vivo in the BAT of WT and TrpA1 KO mice in normal physiological conditions associated with temperature variation. The in vivo assays will be performed at 22 and 30 °C (thermoneutrality). We will determine the temporal oscillation of plasmatic NPs, of clock genes and tissue-specific genes identified by comparative transcriptome using the following methodologies: quantitative PCR, Western blot and NPs ELISA assays. To understand BAT responses to cold challenge will provide strategies to interfere, prevent or minimize possible associated pathological changes such as obesity and cancer cachexia. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SUA-CESPEDES, CRISTHIAN D.; DAVID, DANIELA DANTAS; SOUTO-NETO, JOSE A.; LIMA, OTONIEL GONCALVES; MORAES, MARIA NATHALIA; DE ASSIS, LEONARDO V. MONTEIRO; CASTRUCCI, ANA MARIA DE LAURO. Low Temperature Effect on the Endocrine and Circadian Systems of Adult Danio rerio. FRONTIERS IN PHYSIOLOGY, v. 12, . (18/23043-0, 18/16511-8, 17/26651-9, 18/14728-0, 17/24615-5, 18/23915-8)
LACERDA, JOSE THALLES; GOMES, PATRICIA R. L.; ZANETTI, GIOVANNA; MEZZALIRA, NATHANA; LIMA, OTONIEL G.; DE ASSIS, LEONARDO V. M.; GULER, ALI; CASTRUCCI, ANA MARIA; MORAES, MARIA NATHALIA. Lack of TRPV1 Channel Modulates Mouse Gene Expression and Liver Proteome with Glucose Metabolism Changes. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 23, n. 13, p. 24-pg., . (17/24615-5, 21/01659-2, 14/16412-9, 20/04524-8, 18/23915-8, 18/16511-8, 13/02131-5, 12/50214-4, 18/14728-0, 17/26651-9, 21/09923-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
LIMA, Otoniel Gonçalves de. Cold challenge and its effects on thermosensor canonical processes in BAT and their possible interaction with TRP channels. 2021. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Biociências (IBIOC/SB) São Paulo.

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