Functional activity of blood polymorphonuclear leukocytes as an oxidative stress biomarker in human subjects

• Grant number: 97/02335-5
• Support Opportunities: Research Projects - Thematic Grants
• Start date: March 01, 1998
• End date: January 31, 2003
• Field of knowledge: Biological Sciences - Biochemistry - Biochemistry of Microorganisms
• Principal Investigator: Virginia Berlanga Campos Junqueira
• Grantee: Virginia Berlanga Campos Junqueira
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Pesquisadores principais: Hugo Pequeno Monteiro ; Luiz Roberto Ramos

Abstract

In the present work, we studied the role of polymorphonuclear leukocytes (PMN) in aged individuals and coronary heart diseases (CHD) bearing patients, two physiopathological processes associated with overproductíon of reactive oxygen species (ROS). The effects of antioxidant supplementation on the functional activity of PMN from CHD patients were algo determined. The function of PMNs was evaluated bymeasuring of phagocytosis, killing activity, and ROS production. Luminol amplified chemiluminescence (CL) was used to estimate ROS production by stimulated PMNs. Total cholesterol and the LDL-cholesterol fraction from CHD patients were found to be higher than those recommended, returning to normal levels after antioxidant therapy. PMN CL of CHD patients was found to be higher than the associated control groups. Antioxidant therapy administrated to CHD patients lead to an increase in the killing activity accompanied by a decrease in PMN CL of these subjects. The study also showed that killling activity of PMN from human subjects over 60 years was significantly lower than the activity measured in younger subjects. PMN CL produced after stimulation was found to be positively correlated with the increasing age of human subjects (r=0.946, p<0.01). (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SIMON-GIAVAROTTI‚ K.A.; GIAVAROTTI‚ L.; GOMES‚ L.F.; LIMA‚ A.F.; VERIDIANO‚ A.M.; GARCIA‚ E.A.; MORA‚ O.A.; FERNÁNDEZ‚ V.; VIDELA‚ L.A.; JUNQUEIRA‚ V.B.C.. Enhancement of lindane-induced liver oxidative stress and hepatotoxicity by thyroid hormone is reduced by gadolinium chloride. Free Radical Research, v. 36, n. 10, p. 1033-1039, 2002. (97/02335-5)

GOMES‚ LF; GONÇALVES‚ LM; FONSECA‚ FLA; CELLI‚ CM; VIDELA‚ LA; CHAIMOVICH‚ H.; JUNQUEIRA‚ VBC. β 2-Glycoprotein I (Apolipoprotein H) Modulates Uptake and Endocytosis Associated Chemiluminescence in Rat Kupffer Cells. Free Radical Research, v. 36, n. 7, p. 741-747, 2002. (97/02335-5)

CHAN‚ S.S.; MONTEIRO‚ H.P.; SCHINDLER‚ F.; STERN‚ A.; JUNQUEIRA‚ V.B.C.. α-Tocopherol modulates tyrosine phosphorylation in human neutrophils by inhibition of protein kinase C activity and activation of tyrosine phosphatases. Free Radical Research, v. 35, n. 6, p. 843-856, 2001. (97/02335-5)

GOMES‚ L.F.; ALVES‚ A.F.; SEVANIAN‚ A.; PERES‚ C.A.; CENDOROGLO‚ M.S.; DE MELLO-ALMADA FO‚ C.; QUIRINO‚ L.M.; RAMOS‚ L.R.; JUNQUEIRA‚ V.B.C.. Role of β 2-Glycoprotein I‚ LDL-‚ and Antioxidant Levels in Hypercholesterolemic Elderly Subjects. Antioxidants & Redox Signaling, v. 6, n. 2, p. 237-244, 2004. (97/02335-5)