Management of citrus leprosis

• Grant number: 08/52691-9
• Support Opportunities: Research Projects - Thematic Grants
• Start date: September 01, 2008
• End date: August 31, 2013
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal Investigator: Elliot Watanabe Kitajima
• Grantee: Elliot Watanabe Kitajima
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated research grant(s): 13/09873-7 - Nineteenth Conference of the IOCV (International Organization of Citrus Virologists), AR.EXT; 11/01797-4 - Visit of Dr. Ronald Ochoa to Biological Institute to help the development of research works on identification and characterization of mites, AV.EXT
• Associated scholarship(s): 13/05457-9 - Studies on the evolution of the sweet orange leaf lesions caused by the Citrus leprosis virus C (CiLV-C), BP.IC; 11/14496-2 - Evaluation of the morphological and genomic diversity of different populations of Brevipalpus (Acari: Tenuipalpidae) species transmitting plant viruses and their ability as vector, BP.MS; 11/15604-3 - Influence of weeds and fertilization in the interaction between predatory mites, Brevipalpus phoenicis, leprosis virus and citrus plants, BP.MS; + associated scholarships 10/07365-6 - Monitoring viruliferous mite populations in citrus orchards, BP.IC; 09/13959-9 - Otimization of citrus leprosis management through the interaction of the different components of the pathosystem, BP.PD; 09/08789-7 - Influence of predaceous mites of the family Phytoseiidae on interactions among vector mite, leprosis virus and citrus plant, BP.PD; 08/10642-1 - Genetic variability of orf p29, the putative CiLV coat protein gene., BP.IC - associated scholarships

Abstract

Citrus leprosis, one of the most important diseases for the Brazilian citrus industry, is caused by a virus and efficiently transmitted by a polyphagous and cosmopolitan mite. The control of the disease has been heavily focused on the chemical control of the Brevipalpus phoenicis vector, with high costs to the growers and the environment. These costs could be almost certainly reduced if more adequate IPM practices were used in the field. Several breakthroughs in the studies involving this complex pathosystem have been observed in the last 10 years, but on individual components of the system. Regarding the mite, it has been addressed different aspects of its biology, its dispersion within and between orchards, and the presence of an endosymbiont. The pathogen, Citrus leprosis virus (CiLV), can now be detected through molecular tests in planta and in the mite vector, since its whole genome has been sequenced. Furthermore, due to its genomic information, it received a new taxonomic classification. Studies also addressed the CiLV host range, which turned out to be significantly broader than initially thought and include plants that often occur naturally in citrus orchards. These information, however, have not been used so far to improve the management of the disease, which has spread throughout South and Central America during the last years. New strategies to manage leprosis in orchards should take in consideration not only the presence of the mite in endemic regions - the current strategy - but also, and mainly, the presence of the virus in the plants and the vector, the potential of different types of lesions to serve as sources of inoculum, the use of varietal resistance, the role of alternative hosts on the disease epidemiology, the presence and management of natural enemies etc. Hence, the objective of this project is to increase the knowledge on leprosis and optimize the management of the disease, considering all of the components of the pathosystem as well as their interactions. (AU)

Scientific publications

(17)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

IZQUIERDO CASTILLO, ISIDRO; ZERMENO DIAZ, LUIS FELIPE; MENDEZ, WALTER; OTERO-COLINA, GABRIEL; FREITAS-ASTUA, JULIANA; LOCALI-FABRIS, ELIANE CRISTINA; DE MORAES, GILBERTO JOSE; CALEGARIO, RENATA FAIER; TASSI, ALINE DANIELE; KITAJIMA, ELLIOT W.. Confirmation of the presence of the Citrus leprosis virus C (CiLV-C) in Southern Mexico. TROPICAL PLANT PATHOLOGY, v. 36, n. 6, p. 400-403, NOV-DEC 2011. (08/52691-9)

KITAJIMA, ELLIOT WATANABE; VERLE RODRIGUES, JOSE CARLOS; FREITAS-ASTUA, JULIANA. Lista comentada de plantas ornamentais naturalmente infectadas por vírus transmitidos por ácaros Brevipalpus. Scientia Agricola, v. 67, n. 3, p. 348-371, 2010. (08/52691-9)

RENATA TAKASSUGUI GOMES; ELLIOT WATANABE KITAJIMA; FRANCISCO ANDRÉ OSAMU TANAKA; JOÃO PAULO RODRIGUES MARQUES; BEATRIZ APPEZZATO-DA-GLÓRIA. Anatomia de lesões foliares causadas pelo vírus da Mancha Clorótica do Clerodendrum, transmitido pelo ácaro Brevipalpus phoenicis em diferentes espécies. Summa Phytopathologica, v. 36, n. 4, p. 291-297, 2010-12-00. (00/11805-0, 08/52691-9)

CALEGARIO, RENATA F.; LABATE, MONICA T. V.; PERONI, LUIS A.; STACH-MACHADO, DAGMAR RUTH; ANDRADE, MAXUEL O.; FREITAS-ASTUA, JULIANA; LABATE, CARLOS A.; MACHADO, MARCOS A.; KITAJIMA, ELLIOT W.. In vitro expression and antiserum production against the movement protein of Citrus leprosis virus C (CiLV-C). TROPICAL PLANT PATHOLOGY, v. 37, n. 2, p. 136-141, MAR-APR 2012. (08/52691-9)

NUNES, MARIA A.; NOVELLI, VALDENICE M.; DA CUNHA, BRUNA A.; SOARES, ALEX J.; DE MINEIRO, JEFERSON L. C.; FREITAS-ASTUA, JULIANA; BASTIANEL, MARINES. Survey of the citrus leprosis vector (Brevipalpus yothersi) and phytoseiids in spontaneous plants of an organic citrus orchard. Experimental and Applied Acarology, v. 82, n. 2, SEP 2020. (09/13959-9, 08/52691-9)

RENATA F. CALEGARIO; ELIANE C. LOCALI; DAGMAR R. STACH-MACHADO; LUÍS ANTÔNIO PERONI; RAQUEL CASERTA; RENATO B. SALAROLI; JULIANA FREITAS-ASTÚA; MARCOS A. MACHADO; ELLIOT W. KITAJIMA. Polyclonal antibodies to the putative coat protein of Citrus leprosis virus C expressed in Escherichia coli: production and use in immunodiagnosis. TROPICAL PLANT PATHOLOGY, v. 38, n. 3, p. 188-197, 2013-05-14. (08/52691-9)

DA SILVA, MARCOS ZATTI; SATO, MARIO EIDI; LEITE DE OLIVEIRA, CARLOS AMADEU; NICASTRO, ROBERTO LOMBA. Interspecific interactions involving Neoseiulus californicus (Acari: Phytoseiidae) and Agistemus brasiliensis (Acari: Stigmaeidae) as predators of Brevipalpus phoenicis (Acari: Tenuipalpidae). Experimental and Applied Acarology, v. 65, n. 3, p. 319-329, MAR 2015. (08/52691-9, 13/05829-3, 09/08789-7)

IZQUIERDO CASTILLO, ISIDRO; ZERMENO DIAZ, LUIS FELIPE; MENDEZ, WALTER; OTERO-COLINA, GABRIEL; FREITAS-ASTUA, JULIANA; LOCALI-FABRIS, ELIANE CRISTINA; DE MORAES, GILBERTO JOSE; CALEGARIO, RENATA FAIER; TASSI, ALINE DANIELE; KITAJIMA, ELLIOT W.. Confirmation of the presence of the Citrus leprosis virus C (CiLV-C) in Southern Mexico. ROPICAL PLANT PATHOLOG, v. 36, n. 6, p. 4-pg., 2011-11-01. (08/52691-9)

KITAJIMA, ELLIOT WATANABE; VERLE RODRIGUES, JOSE CARLOS; FREITAS-ASTUA, JULIANA. An annotated list of ornamentals naturally found infected by Brevipalpus mite-transmitted viruses. CIENTIA AGRICOL, v. 67, n. 3, p. 24-pg., 2010-01-01. (08/52691-9)

KITAJIMA, ELLIOT W.; TASSI, ALINE D.; NOVELLI, VALDENICE M.; CACERES, SARA; AGUIRRE, ALCIDES; COSTA, NORMA; DE MORAES, GILBERTO J.; DEMORAES, GJ; PROCTOR, H. Asymmetry in the number of solenidia on tarsi II of Brevipalpus (Acari: Tenuipalpidae) populations from Argentina. ACAROLOGY XIII: PROCEEDINGS OF THE INTERNATIONAL CONGRESS, v. 6, p. 6-pg., 2011-01-01. (08/52691-9)

INUI KISHI, ROSANGELA NAOMI; STACH-MACHADO, DAGMAR; SINGULANI, JUNYA DE LACORTE; DOS SANTOS, CLAUDIA TAVARES; FUSCO-ALMEIDA, ANA MARISA; CILLI, EDUARDO MAFFUD; FREITAS-ASTUA, JULIANA; PICCHI, SIMONE CRISTINA; MACHADO, MARCOS ANTONIO. Evaluation of cytotoxicity features of antimicrobial peptides with potential to control bacterial diseases of citrus. PLoS One, v. 13, n. 9, SEP 7 2018. (08/52691-9, 12/15346-7, 10/11072-4)

CALEGARIO, RENATA F.; LOCALI, ELIANE C.; STACH-MACHADO, DAGMAR R.; PERONI, LUIS ANTONIO; CASERTA, RAQUEL; SALAROLI, RENATO B.; FREITAS-ASTUA, JULIANA; MACHADO, MARCOS A.; KITAJIMA, ELLIOT W.. Polyclonal antibodies to the putative coat protein of Citrus leprosis virus C expressed in Escherichia coli: Production and use in immunodiagnosis. ROPICAL PLANT PATHOLOG, v. 38, n. 3, p. 10-pg., 2013-05-01. (08/52691-9)

MARCOS ZATTI DA SILVA; MÁRIO EIDI SATO; CARLOS AMADEU LEITE DE OLIVEIRA. Diversidade e dinâmica populacional de ácaros em pomar cítrico. Bragantia, v. 71, n. 2, p. 210-218, 2012-07-13. (08/52691-9)

GARITA, L. C.; TASSI, A. D.; CALEGARIO, R. F.; KITAJIMA, E. W.; CARBONELL, S. A. M.; FREITAS-ASTUA, J.. Common Bean: Experimental Indicator Plant for Citrus leprosis virus C and Some Other Cytoplasmic-Type Brevipalpus-Transmitted Viruses. PLANT DISEASE, v. 97, n. 10, p. 1346-1351, OCT 2013. (08/52691-9)

LAURA CRISTINA GARITA; ALINE DANIELE TASSI; RENATA FAIER CALEGARIO; JULIANA FREITAS-ASTÚA; RENATO B. SALAROLI; GERSON O. ROMÃO; ELLIOT W. KITAJIMA. Experimental host range of Citrus leprosis virus C (CiLV-C). TROPICAL PLANT PATHOLOGY, v. 39, n. 1, p. 43-55, 2014-01-31. (11/14496-2, 08/52691-9)

GARITA, LAURA CRISTINA; TASSI, ALINE DANIELE; CALEGARIO, RENATA FAIER; FREITAS-ASTUA, JULIANA; SALAROLI, RENATO B.; ROMAO, GERSON O.; KITAJIMA, ELLIOT W.. Experimental host range of Citrus leprosis virus C (CiLV-C). ROPICAL PLANT PATHOLOG, v. 39, n. 1, p. 13-pg., 2014-01-01. (08/52691-9, 11/14496-2)

ELLIOT WATANABE KITAJIMA; JOSÉ CARLOS VERLE RODRIGUES; JULIANA FREITAS-ASTUA. Lista comentada de plantas ornamentais naturalmente infectadas por vírus transmitidos por ácaros Brevipalpus. Scientia Agricola, v. 67, n. 3, p. 348-371, 2010-00-00. (08/52691-9)