Effects of the anti-inflammatory drugs on oral epithelium and wound healing

• Grant number: 06/00590-9
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2006
• End date: September 30, 2008
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Marilena Chinali Komesu
• Grantee: Marilena Chinali Komesu
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Non-steroidal anti-inflammatory drugs (NSAIDs) are widely used as symptomatic medication for pain relief. 1 in 7 patients with chronic pain disorders receive NSAIDs, and approximately 1 in 5 may use NSAIDs for acute complaints. The selection of the anti-inflammatory drug is based on the clinical experience, patient convenience, side effects and cost. GI-related side effects are the most common complaint from conventional NSAIDs, but they may also be associated to hepatotoxicity, delay in wound healing, and apoptosis in several cell lines. COX-2 selective drugs showed fewer GI complications, but their use may lead to thrombotic risk and myocardial infarction. Many studies present some effects of NSAIDs on wound healing processes, however they presented many controversial results. OBJECTIVES - As both of medications are widely used, the objectives of our experimental study will be to evaluate histologically, histometrically and immunohistochemically, the effects of NSAIDs on epithelial tissues and wound healing to understand the possiblity for clinical problems in treating patients using this medication. METHOD: Wistar rats will be treated daily with conventional NSAID (Diclofenac 3mg/Kg/day) or selective COX-2 inhibitor (Celecoxib- 1mg/Kg/day) for 7 days. Control group will be animals at same age not receiving medication. After this time, skin wounds (4mm diameter), will be performed on shaved backs of treated and control animals under anesthesia. The aninals will be sacrificed 3 and 7 days after surgery. Tissues – oral mucosa and skin, will be prepared and observed under light microscopy. (AU)