Anti-inflammatory, antioxidant and reparative effects of Casearia sylvestris Swartz leaf extract and derivatives in gingival fibroblasts and keratinocytes. In vitro study.

• Grant number: 24/09363-3
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: August 20, 2024
• End date: March 31, 2025
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Joni Augusto Cirelli
• Grantee: Angélica Letícia Reis Pavanelli
• Supervisor: James Deschner
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Institution abroad: Johannes Gutenberg University Mainz (JGU), Germany
• Associated to the scholarship: 22/13096-5 - Evaluation of the effects of Casearia sylvestris Swartz leaf extract and clerodane diterpene in the treatment of experimental periodontitis and induced palate injury in rats, BP.DR

Abstract

Mucogingival surgeries are widely used in Periodontology and Implantology to improve aesthetics and gingival health. Soft tissue grafting has emerged as an effective technique for these purposes, helping to restore gingival contour and thickness, especially around dental implants and in areas of gingival recession. Minimally invasive graft techniques have attracted attention due to their potential to reduce postoperative discomfort, increase aesthetic predictability and accelerate tissue repair processes. Tissue repair involves a complex sequence of molecular events, with several markers such as basic fibroblast growth factor (bFGF), proliferating cell nuclear antigen (PCNA), transforming growth factor beta (TGF-²), and growth factor vascular endothelial (VEGF). . Wound healing in the oral mucosa is considered ideal, but the characteristics that make it efficient are little known and the main difference between the oral mucosa and the skin is in the fibroblasts and keratinocytes. Effective therapies for skin scars are limited, but research based on oral mucosal wound healing holds promise for new treatments. Natural products, including extracts from plants such as Casearia sylvestris Swartz, have shown promise in wound healing and modulating inflammation. In this context, this study aims to investigate the pharmacological activities of C. sylvestris extract (EEtCs), fraction (EFSCs) and the diterpene clerodane (caseargrewiin J) in an in vitro model using gingival fibroblast cells and keratinocytes, through viability and proliferation cellular, RT - qPCR, ELISA, wound healing experiment and evaluation of the NF-kB signaling pathway. The study seeks to complement the in vivo work (Process:2022/13096-5), aiming to contribute to the development of future herbal treatments for palatal lesions and advance pharmacological research in periodontics.