|Support type:||Scholarships in Brazil - Scientific Initiation|
|Effective date (Start):||August 01, 2011|
|Effective date (End):||July 31, 2012|
|Field of knowledge:||Health Sciences - Pharmacy|
|Principal Investigator:||Niege Araçari Jacometti Cardoso Furtado|
|Grantee:||Amanda Henriques Cavalheiro|
|Home Institution:||Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil|
Casearia sylvestris possesses many popular names, but is more popularly known as guaçatonga. This plant is used in folk medicine for many problems, as antimicrobial, anti-inflammatory, among others. Among the isolated and identified constituents from this species are clerodane diterpenes, which display antimicrobial activity. The antimicrobial activity has also been associated with two gallic acid derivatives. The oral microbiota is the one of the most complex of human body. More than 700 bacterial species were detected by molecular methods. One of the most used chemical agents against oral microorganisms that cause dental caries is chlorhexidine. However, when it is used for long periods display side-effects as: staining of teeth and restorative materials, impaired sense of taste, mucous membrane desquamation and burning tongue sensation. Based on the antimicrobial potential of Casearia sylvestris, the aim of this project is to obtain extracts from Casearia sylvestris by different extractive processes (infusion,decoction,maceration and percolation) and different solvent systems (water and absolute ethanol in different concentrations), as well as to obtain essential oil from leaves (using Clevenger apparatus)in order to select the most active extract by antimicrobial activity evaluation against microorganisms which cause dental caries. After selection of the most active extract, it will be incorporated in a mouthwash formulation in order to evaluate the effect on the color of dental restorative material.