Timing effect on biofilm composition and enamel demineralization in situ

• Grant number: 06/01693-6
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2006
• End date: May 31, 2008
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Cinthia Pereira Machado Tabchoury
• Grantee: Cinthia Pereira Machado Tabchoury
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil

Abstract

Dental biofilm exposed in situ to sucrose for 13 days or longer presents high concentration of extracellular polysaccharide (EPS), high lactobacilli counts and low inorganic concentration. However, these changes and their consequences at earlier stages of biofilm formation are unknown. Thus, the aim of this study will be evaluate the microbiological and biochemical composition of dental biofilm formed in the presence of sucrose or glucose + fructose at different periods, in order to observe its dynamic of maturation and its relationship with enamel demineralization. Twelve adult volunteers will wear, for 3 crossover phases of 14 days, an intra-oral palatal appliance containing 6 human enamel blocks, which will be exposed 8 times/day to the following treatments: distilled and deionized water (T1), 10% glucose + 10% fructose solution (T2) or 20% sucrose solution (T3). The biofilm will be collect after 3, 7 and 14 days of formation and evaluate with regard to microbiological and biochemical composition. Microbiological analyses will consist in counts of total microorganisms (TM), total streptococci (TS), mutans streptococci (MS), lactobacilli (LB), %MS/TM, %MS/TS and %LB/TM. The biochemical variables evaluate will be Fluoride (F), Calcium (Ca), Inorganic phosphorus (Pi), intra (IPS) and extracellular (EPS) polysaccharides. In dental specimens, enamel cross-sectional mineral loss will be determined. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VALE‚ GC; TABCHOURY‚ CPM; ARTHUR‚ RA; DEL BEL CURY‚ AA; PAES LEME‚ AF; CURY‚ JA. Temporal relationship between sucrose-associated changes in dental biofilm composition and enamel demineralization. Caries Research, v. 41, n. 5, p. 406-412, 2007. (06/01693-6)