Treatment of class III furcation defects with expanded polytetrafluorethylene membrane associated or not with anorganic bone matrix/synthetic cell-binding peptide: a histologic and histomorphometric study in dogs

• Grant number: 06/03459-0
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2006
• End date: August 31, 2008
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Marcio Fernando de Moraes Grisi
• Grantee: Marcio Fernando de Moraes Grisi
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The unpredictability of the periodontal regenerative treatment of class III furcation defects has stimulated the investigation of alternative therapies to improve the results by using different techniques and biomaterials. A new peptide-enhanced bone graft material (PepGen P15) has been recently developed and has shown significant results regarding tissue neoformation in the tested infra-bony defects. The purpose of this study is to assess the regenerative potential of the new particulate PepGen P15 bone graft in the treatment of class III furcation defects in dogs, either associated or not with the use of PTFE-e membrane. The study will comprise 7 dogs in which class III furcation defects will be created on the mandibular premolars. In the experimental group, the PTFE-e membrane and the PepGen P15 bone graft will be used; in the control group the PTFE-e membrane will be used alone; and in the negative control group (2nd premolars) no biomaterial will be placed. The 3rd and 4th premolars will be randomly assigned to either the experimental or the control group. The defects will be created and filled with an impression material (Impregum F). After 21 days, the defects will be debrided, the roots will be planed with conventional Gracey curettes (Hu-friedy) and the teeth will be subjected to weekly ultrasound prophylaxis and daily cleaning with 0.12% chlorhexidine gluconate during a 15-day period. In the second surgical phase, the bone level on the mesial and distal roots of the 2nd, 3rd and 4th premolars will be demarcated, the membranes and bone grafts will be placed in the respective bone defects and the surgical wounds will be closed with 4.0 silk sutures. The membranes will be retrieved 4 weeks after placement and the dogs will be euthanized 12 weeks after membrane removal. The anatomic pieces were obtained and prepared for histomorphometric analysis to assess the quality of the repair process, the areas of neoformed tissue, absent tissue (voids), epithelium, connective tissue and neoformed bone, as well as the extension of the neoformed cementum in contact with the dentin. The values will be calculated as percentage from the spots demarcated on root surfaces and relative to the total areas of the bifurcation. Data will be submitted to statistical analysis by Mann-Whitney non-parametric test. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SUAID, FLAVIA A.; MACEDO, GUILHERME O.; NOVAES, JR., ARTHUR B.; BORGES, GERMANA J.; SOUZA, SERGIO L. S.; TABA, JR., MARIO; PALIOTO, DANIELA B.; GRISI, MARCIO F. M.. The Bone Formation Capabilities of the Anorganic Bone Matrix-Synthetic Cell-Binding Peptide 15 Grafts in an Animal Periodontal Model: A Histologic and Histomorphometric Study in Dogs. Journal of Periodontology, v. 81, n. 4, p. 594-603, APR 2010. (06/03459-0)