An alfa-1 chain of type I collagen Sp1-binding site polymorphism as a risk factor for the development of urogenital prolapse

• Grant number: 06/03952-9
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2006
• End date: August 31, 2008
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Manoel João Batista Castello Girão
• Grantee: Manoel João Batista Castello Girão
• Host Institution: Departamento de Ginecologia. Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

Genitourinary prolapse is a common problem which can disturb a woman`s quality of life. The definitive treatment for this condition is surgical, but many procedures fail and recurrences are frequent. So we need to improve prevention and treatment. There is evidence that qualitative and quantitative changes in connective tissue, mainly in collagen could result in genitourinary prolapse. Polymorphisms of the gene that encodes type I collagen can affect its expression and this can lead to changes in its function or quantitaty in the pelvic floor. With this study we want to examine the possible influence of a polymorphism in transcription factor Sp-1 binding site in the gene encoding alfa-1 chain of type I collagen on the risk of genitourinary prolapse. The study group will have 100 women with stage III and IV prolapse. The control group will have 200 women with benign gynecological conditions other then stage III and IV prolapse. Blood samples (5 ml) will be taken into tubes containig anticoagulant EDTA. Genomic DNA will be extracted from whole-blood leukocytes using a commercially available kit (GFX). Determination of the polymorphism will de done by polymerase chain reaction using the Master Mix kit, oligonucleotide primers, restriction site enzymes, tubes and agarose gel. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RODRIGUES, ANDREA MOURA; BATISTA CASTELLO GIRAO, MANOEL JOAO; COTRIM GUERREIRO DA SILVA, ISMAEL DALE; FERREIRA SARTORI, MARAIR GRACIO; MARTINS, KARINA DE FALCO; CASTRO, RODRIGO DE AQUINO. COL1A1 Sp1-binding site polymorphism as a risk factor for genital prolapse. INTERNATIONAL UROGYNECOLOGY JOURNAL, v. 19, n. 11, p. 1471-1475, NOV 2008. (06/03952-9)