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Evaluation of performance of the DIAGNOdent device associated with fluorescent dyes in detecting natural smooth surface caries lesions and caries lesions around orthodontic appliances

Grant number: 06/04102-9
Support type:Regular Research Grants
Duration: November 01, 2006 - October 31, 2007
Field of knowledge:Health Sciences - Dentistry - Pediatric Dentistry
Principal Investigator:Fausto Medeiros Mendes
Grantee:Fausto Medeiros Mendes
Home Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil


Despite the DIAGNOdent has presented good results in detecting caries lesions, the device has showed an poor performance to measure minor mineral changes. For this fact, the association of the device with two fluorescent dyes was recently proposed in order to improve the detection of minor mineral loss caused by dental caries. Thus, the aims of the present study will be to evaluate the use of association between the fluorescent dyes with DIAGNOdent : (1) to detect mineral loss in natural smooth surface caries lesions, and (2) to detect artificial caries lesions around orthodontic brackets bonded with different orthodontic cements. For the aim (1), sixty primary teeth were selected. 0.2 mM TMPyP and 4 mM PPIX will be used as the dyes. Natural caries lesions will be measured with the DIAGNOdent, with and without the dyes. Teeth will be sectioned and evaluated with cross-sectional microhardness and polarized light microscopy. For the aim (2), 60 premolars will be selected. Orthodontic brackets will be bonded with 4 different cements. The samples will be submitted to a cariogenic chalange with a demineralizing solution (pH 4.5) during 16 days. After the demineralization, DIAGNOdent and DIAGNOdent associated with the TMPyP will be used to evaluate the caries lesions. Calcium loss will be measured by atomic emission spectroscopy and the lesion depth by light polarized microscopy. Statistical analyses to evaluate the methods performance in detecting natural lesions and caries lesions around brackets will be used. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
RAGGIO, DANIELA PROCIDA; BRAGA, MARIANA MINATEL; RODRIGUES, JONAS ALMEIDA; FREITAS, PATRICIA MOREIRA; PETTOROSSI IMPARATO, JOSE CARLOS; MENDES, FAUSTO MEDEIROS. Reliability and discriminatory power of methods for dental plaque quantification. Journal of Applied Oral Science, v. 18, n. 2, p. 186-193, MAR-APR 2010. Web of Science Citations: 9.
FORNAZARI ALENCAR, CASSIO JOSE; BRAGA, MARIANA MINATEL; DE OLIVEIRA, ELISABETH; NICOLAU, JOSE; MENDES, FAUSTO MEDEIROS. Dye-enhanced laser fluorescence detection of caries lesions around brackets. Lasers in Medical Science, v. 24, n. 6, p. 865-870, NOV 2009. Web of Science Citations: 10.

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