Evaluation of the renin-angiotensin system in adult offspring in response to alterations during the perinatal period

• Grant number: 08/01489-5
• Support Opportunities: Research Projects - Thematic Grants
• Start date: November 01, 2008
• End date: October 31, 2012
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Joel Claudio Heimann
• Grantee: Joel Claudio Heimann
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Pesquisadores principais: Maria Luiza de Morais Barreto de Chaves ; Miriam Sterman Dolnikoff
• Associated scholarship(s): 11/05973-1 - Assessment of placental and systemic inflammatory response in Wistar rats subjected to air pollution during the pre-or gestational periods, BP.PD; 10/18415-4 - Exposure of wistar rats to air pollution before or during gestation: effects on fetus and placenta, BP.DD; 10/12301-7 - Exposure of Wistar rats to air pollution before or during gestation: effects on adult male offspring, BP.PD; + associated scholarships 10/03895-0 - Evaluation of the effect of the litter size on morfo-functional characteristics of adult offspring in Wistar rats, BP.MS; 09/14567-7 - Analysis of microRNAs expression on cardiac hypertrophy induced by thyroid hormone, "in vivo" and "in vitro". role of AT1 receptor, BP.PD; 09/12207-3 - Effects of maternal hypothyroidism in the cardiac Renin Angiotensin System components of rat fetus., BP.IC; 09/02923-3 - Effect of experimental hyperthyroidism, induced in female rats during pregnancy, in the components of Renin-Angiotensin System in offspring., BP.MS - associated scholarships

Abstract

Several studies have observed that alterations during early stages of life may be associated with morphological and functional changes in adulthood. Barker et al. (1989) have shown an inverse relationship between neonatal body weight and cardiovascular mortality in adult humans. Since than many studies were done on that subject. Several animal models with neonatal low body weight were developed. Protein or total undernutrition and salt restriction during pregnancy and uterine decreased blood flow are some of the models of intra-uterine growth restriction. Since some years, a research line is under development in our laboratory in order to unravel the mechanisms of the effect of salt restriction or overload during the perinatal period on neonatal and adult subjects. It was observed in an initial study, that adult offspring of dams fed with high salt diet during pregnancy have higher blood pressure, functional alterations of the renin-angiotensin system and increased renal angiotensin II content. Additional studies have shown a decreased placental mass and decreased myocardial mass and insulin sensitivity in adult offspring of salt restricted dams during pregnancy. To projects are suggested to continue the research line of the effect of high or low salt intake during pregnancy on adult Wistar rats. The main aims of these projects are to verify if there is a participation of the tissue and circulating renin-angiotensin system on morfological and functional myocardial alterations, on insulin resistance and insulin secretion of pancreatic islets in adult offspring of dams fed low or high salt diet during the perinatal period. Low, normal or high-salt diet will be given to Wistar rats during pregnancy. After birth, normal salt diet will be given to all litters. One part of the offspring will be evaluated on the first day of life (body weight, cardiac structure and circulating glucose and insulin). The other part will be evaluated at adulthood (body weight, cardiac structure, blood pressure, circulating glucose and insulin levels, insulin secretion by the pancreatic islets, gene and protein expression of the circulating and tissue renin-angiotensin system, renin and angiotensin I converting enzyme activity). Key Words: Perinatal period, salt, hypothyroidism, hyperthyroidism, myocardium, insulin resistance, renin-angiotensin system. (AU (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LINO, CAROLINE A.; SHIBATA, CAROLINE E. R.; BARRETO-CHAVES, MARIA LUIZA M.. Maternal hyperthyroidism alters the pattern of expression of cardiac renin-angiotensin system components in rat offspring. JOURNAL OF THE RENIN-ANGIOTENSIN-ALDOSTERONE SYSTEM, v. 15, n. 1, p. 52-60, MAR 2014. (08/01489-5)

DINIZ, GABRIELA PLACONA; CREMASCO TAKANO, ANA PAULA; BRUNETO, ERIKA; DA SILVA, FRANCEMILSON GOULART; NUNES, MARIA TEREZA; MORAIS BARRETO-CHAVES, MARIA LUIZA. New insight into the mechanisms associated with the rapid effect of T-3 on AT1R expression. JOURNAL OF MOLECULAR ENDOCRINOLOGY, v. 49, n. 1, p. 11-20, AUG 2012. (06/61523-7, 08/01489-5)