Comparison of fluorescent antibody test and RT-PCR in brain of dogs with canine distemper and genetic characterization of virus strains detected

• Grant number: 06/04924-9
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2007
• End date: January 31, 2010
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Jane Megid
• Grantee: Jane Megid
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Canine distemper is an infectious disease characterized by several clinical symptoms that obligate differencial diagnosis. Fluorescent antibody test has as problem the central nervous system irregular distribution of canine distemper virus. In addition, several virus strain have been described associated to more sensible diagnostic techniques as well as PCR. The aim of this work evaluate the PCR fluoresce comparetively to FAT in post morten diagnosis as well as to sequence the virus strain in relation to vaccinal strains normally used. (AU)