DNA damage induced by metal ions from orthodontic therapy: in vivo and in vitro analyses

• Grant number: 07/00345-7
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2007
• End date: September 30, 2009
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Daniel Araki Ribeiro
• Grantee: Daniel Araki Ribeiro
• Host Institution: Universidade Federal de São Paulo (UNIFESP). Campus Baixada Santista. Santos , SP, Brazil

Abstract

Nowadays, orthodontic therapy plays an important role with respect to restablish stomatognathic system as fas as contributing for esthetic harmonization to patients. Nevertheless, there are few studies that clarify if corrosion products from orthodontic therapy are able to induce genetic damage so far. Herein, the aim of this study will be to evaluate the putatitve genetic damage induced by metal ions discharge from orthodontic therapy by means of single cell gel (comet) and micronucleus assays in vivo and in vitro. For this, the following parameters will be evaluated: 1) DNA damage in buccal cells by the micronucleus assay following nichel-titanium arches and inox steel appliances in vivo; 2) genotoxicity in ordinary human lymphocytes exposed to corrosion eluates from orthodontic appliances by single cell gel (comet) assay in vitro; 3) mutagenicity in human ordinary lymphocytes exposed to corrosion eluates from orthodontic appliances by the micronucleus assay in vitro. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ANGELIERI, FERNANDA; CARLIN, VIVIANE; MARTINS, RENATO A.; RIBEIRO, DANIEL A.. Biomonitoring of mutagenicity and cytotoxicity in patients undergoing fixed orthodontic therapy. AMERICAN JOURNAL OF ORTHODONTICS AND DENTOFACIAL ORTHOPEDICS, v. 139, n. 4, 1, APR 2011. (07/00345-7)

RIBEIRO, D. A.; CAMPOS, R. R.; BERGAMASCHI, C. T.. Chronic renal failure induces genetic instability in multiple organs of Wistar rats. EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, v. 39, n. 4, p. 289-295, APR 2009. (07/01228-4, 07/00345-7)

ANGELIERI, FERNANDA; MARCONDES, JOAO PAULO C.; DE ALMEIDA, DANIELLE CRISTINA; SALVADORI, DAISY M. F.; RIBEIROE, DANIEL A.. Genotoxicity of corrosion eluates obtained from orthodontic brackets in vitro. AMERICAN JOURNAL OF ORTHODONTICS AND DENTOFACIAL ORTHOPEDICS, v. 139, n. 4, p. 504-509, APR 2011. (07/00345-7)

MATSUMOTO, MARIZA A.; CASTANHO, JULIANE; KAWAKAMI, ROBERTO Y.; RIBEIRO, DANIEL A.. Cytogenetical damage in exfoliated oral mucosa cells in elderly people suffering denture stomatitis. GERODONTOLOGY, v. 27, n. 3, p. 183-188, SEP 2010. (07/01228-4, 07/00345-7)

MARTINS, RENATO A.; DA SILVA GOMES, GUILHERME A.; AGUIAR, JR., ODAIR; RIBEIRO, DANIEL A.. Biomonitoring of oral epithelial cells in petrol station attendants: Comparison between buccal mucosa and lateral border of the tongue. Environment International, v. 35, n. 7, p. 1062-1065, OCT 2009. (07/01228-4, 07/00345-7)

RIBEIRO‚ DA; DE OLIVEIRA‚ G.; DE CASTRO‚ GM; ANGELIERI‚ F.. Cytogenetic biomonitoring in patients exposed to dental X-rays: comparison between adults and children. Dentomaxillofacial Radiology, v. 37, n. 7, p. 404-407, 2008. (07/00345-7)

CARLIN, V.; ARTIOLI, A. J.; MATSUMOTO, M. A.; FILHO, H. N.; BORGO, E.; OSHIMA, C. T. F.; RIBEIRO, D. A.. Biomonitoring of DNA damage and cytotoxicity in individuals exposed to cone beam computed tomography. Dentomaxillofacial Radiology, v. 39, n. 5, p. 295-299, JUL 2010. (07/01228-4, 07/00345-7)

ANGELIERI, FERNANDA; CARLIN, VIVIANE; MARTINS, RENATO A.; RIBEIRO, DANIEL A.. Biomonitoring of mutagenicity and cytotoxicity in patients undergoing fixed orthodontic therapy. AMERICAN JOURNAL OF ORTHODONTICS AND DENTOFACIAL ORTHOPEDICS, v. 139, n. 4, p. 6-pg., 2011-04-01. (07/00345-7)