Tripanocidal activity evaluation of crude extract and fractions of Piperaceae plants

• Grant number: 07/02076-3
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2007
• End date: October 31, 2010
• Field of knowledge: Biological Sciences - Parasitology - Protozoology of Parasites
• Principal Investigator: Regina Maria Barretto Cicarelli
• Grantee: Regina Maria Barretto Cicarelli
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Trypanosoma cruzi is the ethiological agent of Chagas disease and transmitted to human by triatomine bugs or through blood transfusion, which affects 16 to 18 million people in Latin America. The therapy continues partially inefficacious despite the efforts of the researchers. In the current literature, there are many investigations on the tripanocida activity for a great variety of natural extracts, especially those from vegetables, or isolated compounds and half-synthetic ones. These analyses can be made through a process of counting of parasites on the microscope, which demanding great time, intensive work in the laboratory and the presence of an unique observer; or using colorimetric methods, as MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] reduction in formazan that is easier and faster application. This colorimetric assay has successfully been used with mammalian cells and also with some protozoa. So, this project focus to evaluate the tripanocida activity of extracts and soluble fractions of plants of the Piperaceae family in epimastigotes forms of Trypanosoma cruzi by using the colorimetric method as an efficient faster test. The samples, which present significant activity, will be also analyzed in phase contrast microscopy for evaluation about the morphologic variations of the parasites. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GABRIELA ALVES LICURSI VIEIRA; MARCO TÚLIO ALVES DA SILVA; LUIS OCTÁVIO REGASINI; FERNANDO COTINGUIBA; HELEN JULIE LAURE; JOSÉ CÉSAR ROSA; MAYSA FURLAN; REGINA MARIA BARRETTO CICARELLI. Trypanosoma cruzi: analysis of two different strains after piplartine treatment. Brazilian Journal of Infectious Diseases, v. 22, n. 3, p. 208-218, 2018-06-00. (07/02076-3, 11/24017-4)

LICURSI VIEIRA, GABRIELA ALVES; ALVES DA SILVA, MARCO TULIO; REGASINI, LUIS OCTAVIO; COTINGUIBA, FERNANDO; LAURE, HELEN JULIE; ROSA, JOSE CESAR; FURLAN, MAYSA; BARRETTO CICARELLI, REGINA MARIA. Trypanosoma cruzi: analysis of two different strains Check for after piplartine treatment. Brazilian Journal of Infectious Diseases, v. 22, n. 3, p. 208-218, MAY-JUN 2018. (11/24017-4, 07/02076-3)