Different modalities of therapy of induced alveolitis in mice: a morphometric and molecular analysis

• Grant number: 07/03301-0
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2007
• End date: January 31, 2009
• Field of knowledge: Health Sciences - Dentistry - Oral and Maxillofacial Surgery
• Principal Investigator: Osny Ferreira Júnior
• Grantee: Osny Ferreira Júnior
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

Alveolitis is a post-surgical infection that affect sockets of recent extracted teeth. It has an incidence between 2 and 4% of exodontics and of almost 25% in partially impacted teeth with pericoronitis.1 Despite of the fact that the alveolitis to harm the alveolar repair process, the alteration on the biological mechanism by this kind of infection is still not known, which raise difficulties in determinate an ideal form of therapy. Therefore, the aim of this work is to elucidate the biological mechanisms involved in the repair process of intentional infected sockets of mice, and compare different modalities of therapy. Ninety six mice will be used in the study, constituting the following groups: Group 1: socket not infected (control group); Group 2: socket infected without any therapy; Group 3: socket infected and treated with irrigation of 2% sodium iodide and 10v hydrogen peroxide solution at 1:1 proportion; and Group 4: socket infected submitted to curettage, physiological sailing and alveolar filling with 10% metronidazol and 2% lidocaine paste, using carboximetilcelulose and mint as therapeutic vehicle. The animals will be divided in the number of 8 per group, which will be sacrificed at 6, 15 and 28 post-surgical days. The obtained specimens from 5 animals of each group will be analyzed by optical microscopy, and the results will be submitted to qualitative and quantitative (histomorfometric) analysis. In addition, it will be performed an expression quantitative analysis of the genes involved in the repair process [type I collagen, VEGF, osteocalcine (OCN), alkaline phosphatase (ALP) and CBFA-1] in the 3 remaining animals of each group, by RealTimePCR, correlating their expression with the histological characteristics observed before. (AU)